中国现代医学杂志2018，Vol.28Issue(1)：44-49,6.DOI:10.3969/j.issn.1005-8982.2018.01.009
干扰素诱导跨膜蛋白3在结肠癌组织中的表达及对结肠癌细胞凋亡的调控作用研究
Expression of IFITM3 in colon cancer tissue and its effect on apoptosis of colon cancer cells
卞俊杰 1李醒亚 2郭伟华 1田国防 1贵永贤 1吕振选1
作者信息
- 1. 河南省新乡市中心医院 肿瘤内科,河南 新乡 453000
- 2. 郑州大学第一附属医院 肿瘤科,河南 郑州 450052
- 折叠
摘要
Abstract
Objective To investigate the expression of interferon-induced transmembrane 3 (IFITM3) in colon cancer tissues and its effect on the apoptosis of colon cancer cells. Methods The expression level of IFITM3 in colon cancer tissues and corresponding adjacent tissues was detected by qRT-PCR. Colon cancer cells were transfected with si-IFITM3, while si-control was used as a control. The proliferation of the transfected cells was detected by MTT. Cell apoptosis was detected by flow cytometry. Western blot was used to detect the expression levels of Bcl-2, Bax, p-STAT3 and STAT3 proteins in the cells. Results The expression level of IFITM3 in the colon carcinoma tissues was significantly higher than that in the adjacent tissues, and the difference was statistically significant (P < 0.05). The survival rates of colon cancer HT29 and HCT116 cells of the si-IFITM3 groups were significantly decreased compared with those of the si-control groups (P < 0.05). The apoptosis rates of colon cancer HT29 and HCT116 cells of the si-IFITM3 groups were significantly higher than those of the si-control groups, and the differences were statistically significant (P < 0.05). The expression levels of STAT3 in the si-IFITM3-transfected HT29 and HCT116 cells did not change compared with those in the si-control groups, but the expressions of p-STAT3 and Bcl-2 protein were significantly lower than those in the si-control groups, and Bax protein expression levels were significantly higher than those in the si-control groups. Conclusions IFITM3 is over-expressed in colon cancer tissues. Inhibition of IFITM3 expression can inhibit the proliferation of colon cancer cells and promote the apoptosis of the cancer cells, its mechanism is related to Bcl-2, Bax and STAT3.关键词
结肠癌/干扰素诱导跨膜蛋白3/凋亡/增殖Key words
colon cancer/IFITM3/apoptosis/proliferation分类
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卞俊杰,李醒亚,郭伟华,田国防,贵永贤,吕振选..干扰素诱导跨膜蛋白3在结肠癌组织中的表达及对结肠癌细胞凋亡的调控作用研究[J].中国现代医学杂志,2018,28(1):44-49,6.
