摘要
Abstract
Objective To determine whether methylation of F2RL3 gene is associated with secondary cardiovascular events and mortality of stable coronary heart disease and with the harmful effect of smoking. Methods A total of 121 inpatients participating cardiovascular rehabilitation programmes after experiencing acute coronary syndrome, myocardial infarction or coronary intervention were recruited in our hospital. Active follow-up was conducted over 8 years. Methylation of F2RL3 gene was characterized by Sequenom matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Associations of methylation of F2RL3 gene and smoking with secondary cardiovascular events, and specific-cause and all-cause mortality were examined by multiple Cox's regression model through estimating confounder-controlled hazard ratios. Results During the follow-up, 5 patients suffered from non-fatal myocardial infarction, 4 had non-fatal strokes, 6 died of cardiovascular diseases, and 5 died due to other causes. After controlling established prognostic factors, Cox's regression analysis revealed F2RL3 methylation was closely associated with mortality. Adjusted hazard ratios (95％ CI) for death from cardiovascular,non-cardiovascular, and any other cause were 2.32 (0.97, 5.58), 5.16 (1.81, 14.7), and 3.19 (1.64, 6.21) in the subjects with the lowest quartile of methylation of F2RL3 gene in comparison to those with the highest quartile. There was no association between the secondary cardiovascular event outcomes of the two groups. The strong associations of smoking with all outcomes were markedly weakened when F2RL3 was included in the regression models. Conclusions The results seem to indicate methylation of F2RL3 is a potential mediator of the detrimental impact of smoking and is strongly related to mortality of patients with stable coronary heart disease.关键词
稳定型冠状动脉粥样硬化性心脏病/甲基化/F2RL3Key words
stable coronary heart disease/epigenetic methylation/F2RL3分类
医药卫生
