卢丽娟 1钟华清 1徐梦华 1苏犁云 1曹凌峰 1董妞妞 1董左权 1徐锦1
作者信息
- 1. 复旦大学附属儿科医院临床检验中心 上海,201102
- 折叠
摘要
Abstract
Objective To characterize the molecular epidemiology of human adenoviruses (HAdVs) and understand their association with acute diarrhea in outpatient children in Shanghai.Methods Fecal samples were collected from children under five years who were admitted to Children's Hospital of Fudan University as outpatients and diagnosed as acute diarrhea (Duration of diarrhea ≤ 14 days) from January 2012 to December 2016.All selected stool samples were subjected to reverse transcriptase PCR (RT-PCR) or PCR to detect group A rotavirus (RVA),human calicivirus (HuCV),human astrovirus (HAstV) and HAdV.Results A total of 1 010 fecal specimens were prospectively enrolled in this study with annual total of 144,144,144,265 and 313 samples collected respectively from 2012 to 2016.Among the enrolled patients,628 of them were boys and 382 were girls.Among them,246 individuals aged 0 to 6 months,578 individuals aged 7 to 12 months,79 individuals aged 13 to 24 months,45 individuals aged 25 to 36 months,18 individuals aged 37 to 48 months and 44 individuals aged 49 to 60 months.①HAdV infections were detected in 5.2％(53/1010) specimens and the detection rate was 6.9％(10/144),10.4％(15/144),5.6％ (8/144),4.5％ (12/265) and 2.6％ (8/313) respectively from 2012 to 2016.And they did not achieve statistical significance (x2=13.436,P=0.09).②There was no significant difference of HAdV detection rate among different gender groups,age groups and each month.③Of the 53 HAdV-positive samples,62.3％ (33/53) were classified as EAdV including HAdV 41 (31/53,58.5％) and-40 (3.8％,2/53) while the others were NEAdV including HAdV-31 (11.3％),-1 (5.7％),-3 (5.7％),-2(3.8％),-5 (3.8％),-6 (3.8％),-4 (1.9％) and-7 (1.9％).HAdV 41 was the most prevalent HAdV genotype in each year.④Among the HAdVs positive specimens,49.1％ (26/53) were EAdV mono-infections and 24.5％ (13/53) were NEAdV monoinfections while others were mixed infection with other diarrhea viruses.Among the mixed-infection cases,HAdVs mixed with RV (13.2％,7/53) or NoV (9.4％,5/53) were the most patterns.Conclusion These results clearly indicated the high diversity of the HAdVs genotypes detected in outpatient children with acute diarrhea and most of them were HAdV mono-infection cases.关键词
门诊/婴幼儿/腹泻/腺病毒/型别Key words
Outpatient/Infants/Diarrhea/Human adenovirus/Genotype
