中国药科大学学报2017，Vol.48Issue(5)：609-613,5.DOI:10.11665/j.issn.1000-5048.20170517
丝素肽对Aβ25-35致SH-SY5Y细胞损伤的保护作用及机制研究
Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism
摘要
Abstract
This study aimed at exploring the molecular mechanism of fibroin peptide in preventing the Aβ25-35-induced neuronal damages in SH-SY5Y cells.MTT was used to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced injuries in SH-SY5Y cells;Western blot was employed to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced hyperphosphorylation of Tau in SH-SY5Y cells;DCFH-DA probe method was used to detect the effect of fibroin peptide on the Aβ-induced production of intracellular reactive oxygen species (ROS) in SH-SY5Y cells.The results indicated that fibroin peptide could improve the activity of the PP2A and inhibit the activity of GSK-3β to decrease the hyperphosphorylation of Tau induced by Aβ25-35.Fibroin peptide could significantly prevent the Aβ25-35-induced neuronal damages and multisite Tau hyperphosphorylation.In addition,fibroin peptide could also reduce oxidative damage to protect neurons by significantly decreasing the Aβ25-35-induced production of intracellular ROS.关键词
丝素肽/阿尔茨海默病/神经保护活性/Tau蛋白磷酸化/活性氧Key words
fibroin peptide/Alzheimer' s disease/neuroprotective effect/Tau phosphorylation/reactive oxy gen species分类
医药卫生引用本文复制引用
尉海洋,陈松,徐政,高向东,姚文兵..丝素肽对Aβ25-35致SH-SY5Y细胞损伤的保护作用及机制研究[J].中国药科大学学报,2017,48(5):609-613,5.基金项目
国家自然科学基金资助项目(No.81473216)This study was supported by the National Natural Science Foundation of China(No.81473216) （No.81473216）
