中国药理学通报2018，Vol.34Issue(1)：23-27,5.DOI:10.3969/j.issn.1001-1978.2018.01.007
眼镜蛇毒NGF通过PI3K/Akt促人肝星状细胞凋亡的机制研究
Effect of cobra venom NGF on inducing apoptosis of LX2 cells by inhibiting Akt signaling pathway and its mechanism
摘要
Abstract
Aim To study the effect of cobra venom nerve growth factor ( NGF) on inducing the apoptosis of LX2 cells, the key cells of hepatic fibrosis , through Akt signaling pathway and its underlying mechanism .Methods CCK-8 method was used to detect the pro-liferation of LX2 cells at different concentrations of NGF and LY294002 .Flow cytometry was applied to detect the effect of NGF on the apoptosis of LX 2 cells. Western blot was used to study the effects of NGF and LY294002 respectively , and their combination on the p-Akt protein level .Results NGF could decrease the survival rate of LX2 cells, and the minimum effective concentration was 1mg· L-1; it increased the apopto-sis rate of LX2 cells within the rise of concentration un-der a certain of range and decreased the expression level of p-Akt, but it had no significant effect on the ex-pression of Akt .Conclusions NGF may promote the apoptosis of LX2 cells by inhibiting the activation of PI3K/Akt signaling pathway in a concentration-de-pendent manner .The study of the pathogenesis of liver fibrosis is significant for the clinical treatment of liver fibrosis.关键词
肝纤维化/人肝星状细胞/眼镜蛇毒/神经生长因子/凋亡/PI3K/AktKey words
hepatic fibrosis/human hepatic stellate cell/cobra venom/nerve growth factor/apoptosis/PI3 K/Akt分类
医药卫生引用本文复制引用
蔡凤桃,张学荣,孙林,王秀男,廖明,班建东,陈缨,农君..眼镜蛇毒NGF通过PI3K/Akt促人肝星状细胞凋亡的机制研究[J].中国药理学通报,2018,34(1):23-27,5.基金项目
国家自然科学基金资助项目(No81360078, 81460128) （No81360078, 81460128）
