中国药理学通报2018，Vol.34Issue(1)：27-32,6.DOI:10.3969/j.issn.1001-1978.2018.01.008
人参皂苷CK对人肝癌细胞HepG2迁移及侵袭的影响
Effect of ginsenoside metabolite compound K on migration and invasion of human hepatocellular carcinoma line HepG2
摘要
Abstract
Aim To investigate the effect of ginsen-oside metabolite compound K ( CK) on migration and invasion of human hepatocellular carcinoma line HepG2, and the possible signaling pathway underlying these processes .Methods HepG2 cells were exposed to ginsenoside CK (0, 10, 20, 40, 80 μmol· L-1 ) for 24 h.The cell viability was examined by MTT as-say, and the ability of migration and invasion was ob-served with the wound healing and transwell assay .The expression of E-cadherin , N-cadherin and other related signal molecules such as p-ERK, ERK, p-Akt, Akt were detected by Western blot .Results The cell via-bility was significantly reduced by ginsenoside CK (20, 40, 80 μmol· L-1) (P<0.01).The ability of cell migration and invasion was significantly inhibited after exposure to ginsenoside CK .After treatment with ginsenoside CK (20, 40, 80 μmol · L-1 ) in HepG2 cells, the expression of E-cadherin markedly in-creased, while N-cadherin expression significantly de-creased.Meanwhile, the expression of p-ERK and p-Akt decreased after treated with ginsenoside CK .Con-clusion Ginsenoside CK inhibits the migration and invasion of human hepatocellular carcinoma line HepG2, which may be through suppression of ERK and Akt signaling .关键词
人参皂苷CK/HepG2细胞/细胞活力/细胞迁移/细胞侵袭/上皮间质转化Key words
ginsenoside CK/HepG2 cell/cell viabili-ty/migration/invasion/epithelial mesenchymal transi-tion分类
医药卫生引用本文复制引用
彭文婷,孙妩弋,孙家昌,杜佳佳,魏伟..人参皂苷CK对人肝癌细胞HepG2迁移及侵袭的影响[J].中国药理学通报,2018,34(1):27-32,6.基金项目
国家自然科学基金资助项目(No81770605,81300332) （No81770605,81300332）
