中国药理学通报2018，Vol.34Issue(1)：97-102,6.DOI:10.3969/j.issn.1001-1978.2018.01.021
丹酚酸B通过PPARα抑制2型糖尿病小鼠心肌肥厚
Salvianolic acid B ameliorates cardiac hypertrophy in T2 DM mice by activating PPARα
摘要
Abstract
Aim To investigate the protective role of salvianolic acid B ( Sal B ) on cardiac hypertrophy in type 2 diabetes mice , and to explore its influence on peroxisome proliferator activated receptors-α( PPARα) .Methods The type 2 diabetes melitus ( T2DM) mouse model was established by 4 weeks ' high fat diets feeding and one time STZ intraperitoneal injection .The animals were randomly divided into:control, T2DM, T2DM+SalB(100 mg· kg -1 · d-1 ) and Sal B(100 mg· kg -1 · d-1 ) groups.Eight weeks later, heart weight, tibial length, cross section area of cardiomyocytes , protein expression of PPARαin heart tissue were recorded .In vitro, high glucose and high insulin ( HGI ) were used to induce hypertrophic growth in cultured neonatal rat cardiomyocytes ( NRC-Ms) .And cell surface area , 3 H-leucine incorporation , 3 H-D-glucose uptake and PPARαprotein level were measured to observe the effect of Sal B and MK 886, a PPARαinhibitor.Results In T2DM model mice, Sal B could lower heart weight/tibial length and cross sec-tion area of cardiomyocytes , while PPARαprotein level in hearts were improved .In cultured cardiomyocytes , Sal B ( 10 ～100 μmol · L-1 ) ameliorated the in-creased levels of cell surface area ,3 H-leucine incorpo-ration and improved the decreased 3 H-D-glucose up-take and PPARαexpression induced by HGI . But those function could be abolished by MK 886.Conclu-sion Sal B ameliorates cardiac hypertrophy in T 2DM mice, which may be related to its function on PPARαactivation .关键词
丹酚酸B/2型糖尿病/心肌肥厚/高糖/高胰岛素/过氧化物酶体增殖物激活受体αKey words
salvianolic acid B/type 2 diabetes/cardi-ac hypertrophy/high glucose/high insulin/peroxi-some proliferator activated receptor-α.分类
医药卫生引用本文复制引用
臧慧梅,苏磊,陈家政,闫涛,林家茂,房丽君..丹酚酸B通过PPARα抑制2型糖尿病小鼠心肌肥厚[J].中国药理学通报,2018,34(1):97-102,6.基金项目
山东省自然科学基金资助项目(No2016ZRC03060) （No2016ZRC03060）
