中国药理学通报2018，Vol.34Issue(1)：118-122,5.DOI:10.3969/j.issn.1001-1978.2018.01.025
山茱萸环烯醚萜苷对D-GalN/TNF-α损伤肝细胞的保护作用研究
Protective effect of cornel iridoid glycoside on hepatocytes injured by D-galactosamine/tumor necrosis factor-α
摘要
Abstract
Aim To determine the effect of cornel iri-doid glycoside ( CIG ) on human hepatocyte cell line (L-02) injured by D-galactosamine (GalN) and tumor necrosis factor-α( TNF-α) .Methods Firstly, CIG was extracted , separated and purified . Cell lesion model injured by D-GalN/TNF-αwas tested by MTT method.T-AOC, SOD, MDA and calcium ion concen-tration were taken as indicators to study the effects of CIG on L-02 cell injured by D-GalN/TNF-α.The ex-pression of p-PERK, p-eIF-2α, caspase-3 protein were detected by Western blot .Results 44 mg · L-1 D-GalN and 100 μg · L-1 TNF-αwere suitable for L-02 cell lesion model.CIG high, middle, low concentra-tion group could significantly increase the L-02 cell ac-tivity by 21％, 13％, 8％, respectively and SOD activity and T-AOC ability as well compared with model group.At the same time, they markedly reduced the MDA activity except the low concentration .Three con-centrations of CIG could reduce the expression of endo-plasmic reticulum stress related protein PERK , eIF-2αand apoptosis-associated protein caspase-3. Conclu-sions CIG could protect L-02 cells injured by D-GalN/TNF-α.Increasing the cellular antioxidant abili-ty, reducing the damage of endoplasmic reticulum stress and the expression of apoptosis-associated protein may be the possible mechanism .关键词
山茱萸环烯醚萜苷/D-氨基半乳糖/肿瘤坏死因子α/肝损伤/抗氧化/内质网应激/肝保护Key words
cornel iri doid glycoside/D-galac-tosamine/tumor necrosis factor-α/liver damage/anti-oxidation/endoplasmic reticulum stress/live protection分类
医药卫生引用本文复制引用
马艳霞,吴勉华,姜泽群,赵凤鸣,李黎,李沐涵,陆兔林,杭爱武..山茱萸环烯醚萜苷对D-GalN/TNF-α损伤肝细胞的保护作用研究[J].中国药理学通报,2018,34(1):118-122,5.基金项目
国家自然科学基金资助项目(No81373971,81503535) （No81373971,81503535）
江苏省高校自然科学基金资助项目(No13KJB60010) （No13KJB60010）
