中国医学科学院学报2017，Vol.39Issue(5)：611-614,4.DOI:10.3881/j.issn.1000-503X.2017.05.003
PEI-Fe3O4纳米颗粒标记人羊膜上皮细胞
PEI-Fe3O4 Nanoparticles for Human Amniotic Epithelial Cells Labeling
摘要
Abstract
Objective To label human amniotic epithelial cells (hAECs) by using PEI-Fe3O4 nanoparticles.Methods The PEI-Fe3O4 nanoparticles were characterized by using transmission electron microscopy and dynamic light scattering.The primary cultured hAECs were labeled with the nanoparticles,and the labeling efficiency was evaluated by Prussian blue staining.The cell survival rate and viability were tested by using placenta blue staining and CCK-8 assay,respectively.Results The PEI-Fe3O4 nanoparticles were compact spheres with an average particle size of 13 nm,a hydrodynamic radius of 17.56 nm,and a zeta potential of + 34.5 mV.The labeling efficiency of the nanoparticles on hAECs reached 91％ when the concentrations were greater than 20 μg/ml.When the concentrations of nanoparticles were at 50 μg/ml (t =16.37,P ＜ 0.0001;t =10.39,P ＜ 0.0001) and 100 μg/ml (t =29.89,P ＜ 0.0001;t =16.86,P ＜ 0.0001),the cell survival rates and cell viabilities were significantly reduced versus controls.Conclusion The PEI-Fe3O4 nanoparticles can be used for labeling hAECs without obvious cytotoxicity at its working concentration.关键词
PEI-Fe3O4纳米颗粒/人羊膜上皮细胞/标记Key words
PEI-Fe3O4 nanoparticles/human amniotic epithelial cells/labeling分类
生物科学引用本文复制引用
张殿宝,李莹,苏航,王竟,施萍,庞希宁..PEI-Fe3O4纳米颗粒标记人羊膜上皮细胞[J].中国医学科学院学报,2017,39(5):611-614,4.基金项目
国家重点基础研究发展计划(2012CB518103)、辽宁省医疗器械研发项目(2014305012)、沈阳市干细胞与再生医学重点实验室项目(F15-157-1-00)、沈阳市科学技术计划项目(F14-201-4-00)和中国医科大学科研基金(XZR20160022) Supported by the National Basic Research Program of China (2012CB518103),the Medical Equipment Research and Development Project of Liaoning Province (2014305012),Shenyang Key Laboratory Project (F15-157-1-00),the Science and Technology Planning Project of Shenyang (F14-201-4-00),and the Foundation of China Medical University (XZR20160022) （2012CB518103）
