植物学报2017，Vol.52Issue(6)：713-722,10.DOI:10.11983/CBB16239
甘蓝型油菜BnTTG1-1基因的功能分析
Functional Analysis of Brassica napus BnTTG1-1 Gene
摘要
Abstract
AtTTG1 existing in the nucleus as a WD40 repeat transcription factor plays important roles in regulating trichome initiation,anthocyanin biosynthesis,and storage reserve accumulation in Arabidopsis thaliana.In the present study,we cloned the full-length coding domain sequence (CDS) of the BnTTG1-1 gene from the Brassica napus cv.‘QINYOU Seven',analyzed its subcellular localization,detected its temporal and spatial expression patterns in different tissues,and investigated its functions in several biological processes.BnTTG1-1 was localized in the nucleus of tobacco leaf cells,so it may function as a transcription factor.BnTTG1-1 was widely expressed in various vegetative tissues and developing seeds in QINYOU Seven.Moreover,introducing 35S:BnTTG1-1 into the mutant ttg1-13 fully rescued many phenotypes of the mutant,such as no trichomes and anthocyanins,yellow seed coat,higher contents of seed fatty acids and storage proteins,and sensitivity to higher sucrose or salinity stresses during seed germination and seedling establishment.Thus,BnTTG1-1 and AtTTG1 exhibited conserved functions on many biological processes during plant growth and development.关键词
TTG1/甘蓝型油菜/拟南芥/功能互补Key words
TTG1/Brassica napus/Arabidopsis thaliana/functional complementation引用本文复制引用
刘凯歌,齐双慧,段绍伟,李东,金倡宇,高晨浩,刘绚霞,陈明训..甘蓝型油菜BnTTG1-1基因的功能分析[J].植物学报,2017,52(6):713-722,10.基金项目
国家自然科学基金(No.31501336)、西北农林科技大学引进人才启动经费(No.Z111021402)和优青培养科研专项(No.Z109021517) （No.31501336）
