植物学报2017，Vol.52Issue(6)：723-732,10.DOI:10.11983/CBB16237
山葡萄种质资源DNA条形码通用序列的筛选
Screening of Universal DNA Barcodes for Vitis amurensis
摘要
Abstract
To determine the best candidate sequence that can be used as a universal DNA barcode to identify Vitis amurensis varieties,we used PCR amplification with the ideal annealing temperature and sequenced the DNA fragments of five different regions (ITS,ITS2,psbA-tmH,rbcL and matK) of Amur grape samples.The effective sequence ratios obtained by PCR amplification and sequencing,divergence of intra-and inter-varieties,DNA barcoding gap and identification by using BLAST and NJ tree methods were used to evaluate the discrimination ability of these candidate sequences.ITS2 and psbA-tmH had fairly high amplification efficiency and sequencing efficiency in 33 samples belonging to 11 V.amurensis resources;they had larger divergences of intra-and inter-varieties and better DNA barcoding gaps than the other regions tested (ITS,rbcL and matK).In addition,ITS2 could discriminate some varieties that could not be successfully discriminated by the psbA-tmH region.ITS2 and psbA-tmH may be promising DNA barcode region combinations for identifying V.amurensis resources.DNA barcoding can compensate for morphological identification and help in the exact identification of V.amurensis resources.关键词
山葡萄/DNA条形码/鉴定/ITS2/psbA-trnH/序列Key words
Vitis amurensis/DNA barcode/identification/ITS2/psbA-trnH/sequence引用本文复制引用
宋慧芳,刘海双,杨义明,范书田,李昌禹,艾军..山葡萄种质资源DNA条形码通用序列的筛选[J].植物学报,2017,52(6):723-732,10.基金项目
农业部物种资源保护(农作物)项目(No.2016NWB043)和国家现代农业产业技术体系建设专项(No.CARS-30-7) （农作物）
