中国中西医结合杂志2017，Vol.37Issue(12)：1447-1450,4.DOI:10.7661/j.cjim.20170828.246
参地颗粒对慢性肾小球肾炎脾肾亏虚证患者T细胞亚群及足细胞标志蛋白的影响
Effect of Shendi Granule on T Cell Subsets end Podocyte Marker Protein in Chronic Glomerulonephritis Patients with Pi-Shen Deficiency Syndrome
摘要
Abstract
Objective To study the mechanism of Shendi Granule (SDG) on regulating cellular immunity and protecting podocytes in chronic glomerulonephritis (CGN) patients with Pi-Shen deficiency syndrome (PSDS).Methods Totally 60 CGN patients with PSDS were randomly assigned to the control group and the treatment group,30 in each group.Five patients were excluded (3 patients in the treatment group and 2 patients in the control group),and 55 patients completed the trial (28 cases in the control group and 27 cases in the treatment group).Besides,a healthy normal group (20 cases) was set up.Patients in the control group took Valsartan Capsule (VC),80 mg each time,once per day.Patients in the treatment group took SDG,1 package each time (10 g),3 times a day.The treatment course for all was 12 weeks.The clinical efficacy was observed in the two groups.24 h urinary protein quantization,levels of whole blood CD4+T and CD8+T cells,serum levels of IL-2,IL-4,IL-17,urinary levels of podocalyxin (PCX) and B7-1 were detected in the two groups before and after treatment.Results The total effective rate and total effective rate of TCM syndrome were better in the treatment group than in the control group (P＜0.05).After treatment 24 h urinary protein quantization could be reduced in the two groups (P ＜0.05).It was better in the treatment group than in the control group (P ＜0.05).Before treatment whole blood CD4 +T level,CD4+T/CD8+T ratio,and serum IL-4 level were lower in the two groups than in the normal group (P ＜0.05).Whole blood CD8+T level,serum levels of IL-2,IL-4,IL-17,and urinary levels of PCX and B7-1 were higher in the two groups than in the normal group (P ＜0.05).After treatment whole blood CD4 +T level,CD4 +T/CD8 +T ratio,and serum IL-4 level increased more in the treatment group and the control group,as compared with before treatment (P ＜0.05).The increase was superior in the treatment group than in the control group (P ＜0.05).Whole blood CD8 +T level,serum levels of IL-2 and IL-17,and urinary levels of PCX and B7-1 decreased more in the treatment group and the control group after treatment than before treatment (P ＜0.05).The decrease was superior in the treatment group than in the control group (P ＜0.05).Conclusions SDG could obviously ameliorate clinical symptoms in CGN patients with PSDS,and reduce 24 h urinary protein quantization.Its mechanism might be associated with correcting imbalanced T cell subsets,regulating serum levels of IL-2,IL-4,and IL-17,and urinary levels of PCX and B7-1.关键词
慢性肾小球肾炎/脾肾亏虚证/参地颗粒/T细胞亚群/足细胞标志蛋白Key words
chronic glomerulonephritis/Pi-Shen deficiency syndrome/Shendi Granule/T cell subsets/podocyte marker protein引用本文复制引用
王亿平,张磊,王东,吕勇,胡顺金,刘家生,金华,茅燕萍,任克军..参地颗粒对慢性肾小球肾炎脾肾亏虚证患者T细胞亚群及足细胞标志蛋白的影响[J].中国中西医结合杂志,2017,37(12):1447-1450,4.基金项目
安徽省卫生厅中医药科研项目(No.2012ZY13) （No.2012ZY13）
安徽省卫计委中医药科研项目(No.2016ZY13) （No.2016ZY13）
