中国中西医结合杂志2017，Vol.37Issue(12)：1455-1460,6.DOI:10.7661/j.cjim.20171026.255
低频电针对多囊卵巢综合征大鼠卵巢组织Bax、Bcl-2表达的影响
Effects of Low Frequency Electroacupuncture on the Expressions of Bax and Bcl-2 in the Ovary Tissue of Polycystic Ovary Syndrome Rats
摘要
Abstract
Objective To observe the effects of low frequency electroacupuncture (LF-EA) on the expressions of Bcl-2 associated X protein (Bax),B-cell lymphoma-2 (Bcl-2) in the ovary tissue of polycystic ovary syndrome (PCOS) rats.Methods Totally 24 female 3 weeks old Sprague-Dawley (SD)rats were randomly divided into three groups,the normal control group(C),the model group (M),and the EA group(A),8 in each group.Rats in the M group and the A group were fed with high-fat diet and injected with andronate for 8 weeks.Rats in the A group received LF-EA for 5 successive weeks,30 min each time 5 times per week from 11 weeks old.Rats in the M group and the C group received no intervention.Levels of testosterone (T),fasting insulin (FINS),and fasting blood glucose (FBG) were detected before and after intervention.HOMA-IR was compared.The structure of the ovarian tissue was observed by HE staining.mRNA and protein expressions of Bax and Bcl-2 were measured via real-time fluorescent quantitative PCR and Western blot.Bax/Bcl-2 of the ovarian tissue was calculated.Results The weight of the M group was heavier than that of the C group from week 8 (P ＜0.05,P ＜0.01).The weight of the A group was lighter than that of the M group from week 14 (P ＜0.05).The levels of serum T and FINS,as well as HOMA-IR significantly increased in the M group than those of the C group at week 11 and week 16 (P ＜0.01).The levels of serum T and FINS,as well as HOMA-IR significantly decreased in the A group than those of the M group at week 16 (P ＜0.05,P ＜0.01).HE staining of the ovarian tissue showed some corpora lutea existed in the A group,with lesser cystic follicles.The granular cell layer was obviously thicker in the A group than in the M group.mRNA and protein expressions of Bax were higher in the M group than in the C group;those of Bcl-2 were lower in the M group than in the C group (P ＜0.05,P ＜0.01).mRNA and protein expressions of Bax were lower in the A group than in the M group (P ＜0.05);Bax/Bcl-2 ratio was lower in the A group than in the M group (P ＜0.01).Conclusion LF-EA might inhibit apoptosis of ovarian granulosa cells,improve local ovarian miroenvironment,promote luteal production,and restore normal ovulatory cycle possibly by down-regulating mRNA and protein expressions of Bax and decreasing Bax/Bcl-2 ratio.关键词
多囊卵巢综合征/电针/Bcl-2相关X蛋白/B淋巴细胞瘤-2Key words
polycystic ovary syndrome/electroacupuncture/Bcl-2 associated X protein/B-cell lymphoma-2引用本文复制引用
郑艳华,吴婉婷,马红霞,李娟..低频电针对多囊卵巢综合征大鼠卵巢组织Bax、Bcl-2表达的影响[J].中国中西医结合杂志,2017,37(12):1455-1460,6.基金项目
国家自然科学基金资助项目(No.81704107) （No.81704107）
广东省自然科学基金资助项目(No.2014A030313496) （No.2014A030313496）
