中国中西医结合杂志2017，Vol.37Issue(12)：1466-1470,5.DOI:10.7661/j.cjim.20170908.247
芪参益气滴丸抑制大鼠心肌梗死后心肌纤维化及对TGF-β1/Smads通路表达的影响
Qishen Yiqi Dripping Pill Inhibited Myocardial Fibrosis after Myocardial Infarction in Rats and Its Effects on TGF-β1/Smads Pathway
摘要
Abstract
Objective To observe the effect of Qishen Yiqi Dripping Pill (QYDP) on myocardial fibrosis (MF) after myocardial infarction (MI) in rats,and to study its mechanism based on TGF-β1/Smads pathway.Methods The left anterior descending coronary artery was ligated to establish MI model in male Wistar rats.The successful MI modeling rats were randomly divided into four groups,i.e.,the model group,the conventional dose QYDP group (QSYQ-N),the high dose QYDP group (QSYQ-H),the Captopril group,10 in each group.Besides,a sham-operation group was also set up (n =10).The heart tissue was stained by HE staining and Masson staining after 4 weeks intervention,and collagen volume fraction (CVF) was calculated.mRNA and protein expression levels of transforming growth factor-β1 (TGF-β1),Smad2 and Smad7 in cardiac tissue were detected by Real-time quantitative PCR and Western blot.Results HE staining showed a large area of connective tissues had formed in ventricular wall of the model group.The ventricular wall was obviously thinned.Myocardial cells were obviously dropsy and a large amount of inflammatory cells were infiltrated.The area of ventricular wall connective tissue area was reduced,cell edema and inflammatory cells were attenuated in each treatment group.Masson staining showed that after modeling,MI occurred in rats of each group.Compared with the model group,CVF significantly decreased in each treatment group (P ＜0.01).Compared with the sham-operation group,mRNA and protein expressions of TGF-β1 and Smad2 were significantly increased,Smad7 expression significantly decreased in the model group (P ＜0.05,P ＜0.01).Compared with the model group,mRNA and protein expressions of TGF-β1 and Smad2 were significantly down-regulated,Smad7 expression was significantly up-regulated in each treatment group (P ＜0.05,P ＜0.01),in the QSYQ-H group and the captopril group.Conclusion QSYQ could effectively inhibit MF rats after myocardial infarction,and its mechanism might be related to regulating expressions of related gene and protein through TGF-β1/Smads pathways.关键词
芪参益气滴丸/心肌纤维化/TGF-β1/Smads通路/大鼠Key words
Qishen Yiqi Dripping Pill/myocardial fibrosis/TGF-β1/Smads pathway/rat引用本文复制引用
赵桂峰,毛静远,吴丽玉,黄欣玮..芪参益气滴丸抑制大鼠心肌梗死后心肌纤维化及对TGF-β1/Smads通路表达的影响[J].中国中西医结合杂志,2017,37(12):1466-1470,5.基金项目
教育部“创新团队发展计划”基金资助项目(No.IRT_16R54) （No.IRT_16R54）
天津市科技计划基金资助项目(No.15ZXLCSY00020) （No.15ZXLCSY00020）
天津市高等学校科技发展基金计划资助项目(No.20130218) （No.20130218）
