中国中西医结合杂志2017，Vol.37Issue(12)：1471-1475,5.DOI:10.7661/j.cjim.20170901.260
天香丹对动脉粥样硬化秽浊痰阻证ApoE(-/-)小鼠血清炎性细胞因子及NF-κB p65信号通路的影响
Effect of Tianxiangdan on Inflammatory Factor and NF-κB p65 Signal Pathway of Atherosclerosis ApoE(-/-) Mica with Phlegm Turbidity Obstruction Syndrome
摘要
Abstract
Objective To observe the effect of Tianxiangdan (TXD) on expressions of IL-1 β,tumor necrosis factor-α (TNF-α),and nuclear factor-κB (NF-κB) p65 in apolipoprotein E gene knock-out [ApoE(-/-)] atherosclerosis (AS) mice with phlegm turbidity obstruction syndrome (PTOS),and to explore the possible mechanism of TXD for prevention and treatment of AS.Methods A total of 48 ApoE(-/-) mice (8 weeks old) were divided into the normal control group (A,n =12) and the intervention group (B,n =36).Mice in the normal control group were fed with common diet at room temperature,while those in Group B were intervened in artificial complex climate box.After 12 weeks feeding,AS model with PTOS were successfully duplicated in mice.Then they were divided into three groups,the model group,the TXD group,and the Atorvastatin group.Mice in each group were scarified 12 weeks later.Serum levels of IL-1β and TNF-α in each group were detected.Furthermore,the expression level of NF-κB p65 in aortic tissue was detected by Western blot.Results Levels of serum IL-1β and TNF-α in the model group were significantly higher than those in the normal control group (P ＜0.01).Compared with the model group,serum levels of IL-1 β and TNF-α were reduced obviously in the TXD group and the Atorvastatin group (P ＜0.01).No statistical difference in serum levels of IL-1 β and TNF-α existed between the TXD group and the Atorvastatin group (P ＞0.05).Furthermore,phosphorylation level of NF-κB p65 protein in aortic tissue was higher in the model group than in the normal control group (P ＜0.01).The phosphorylation level of NF-κB p65 protein was lower in the Atorvastatin group and the TXD group than in the model group (P ＜0.01).No statistical difference in the phosphorylation level of NF-κB p65 protein existed between the TXD group and the Atorvastatin group (P ＞0.05).Conclusion The mechanism of TXD in the prevention and treatment of AS might be associated with suppressing the activation of NF-κB p65 signaling pathways,and down-regulating expression levels of inflammatory cytokines IL-1β and TNF-α,thus further inhibiting inflammatory reactions.关键词
天香丹/动脉粥样硬化/炎症反应/核因子-κBKey words
Tianxiangdan/atherosclerosis/inflammatory factor/nuclear factor-κB引用本文复制引用
孙龙飞,古丽加玛力·尼亚孜,马文慧,郭龙龙,安冬青..天香丹对动脉粥样硬化秽浊痰阻证ApoE(-/-)小鼠血清炎性细胞因子及NF-κB p65信号通路的影响[J].中国中西医结合杂志,2017,37(12):1471-1475,5.基金项目
国家自然科学基金资助项目(No.81160428,No.81360571) （No.81160428,No.81360571）
