组织工程与重建外科杂志2017，Vol.13Issue(6)：318-321,4.DOI:10.3969/j.issn.1673-0364.2017.06.004
bFGF对hBMSCs增殖及成软骨能力影响的实验研究
Experimental Study of the Impacts of bFGF on Proliferation and Chondrogenesis of Human Bone Marrow Stromal Cells
赵丹丹 1陶然 2刘豫 3曹谊林 2周广东3
作者信息
- 1. 261042 山东省潍坊市 潍坊医学院整形外科研究所
- 2. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科,上海市组织工程研究重点实验室
- 3. 200241 上海市 组织工程国家工程研究中心
- 折叠
摘要
Abstract
Objective To explore the effect of basic fibroblast growth factor (bFGF) on the proliferation and chondrogenesis of human bone marrow mesenchymal stem cells (hBMSCs), and to determine the cell generation schedule suitable for in vitro cartilage construction of hBMSCs. Methods hBMSCs were obtained and cultured respectively in two culture systems:DMEM and DMEM+bFGF. Two groups of cells were repeatedly passaged to P4 generation, the morphological changes and cell yield rates of hBMSCs were compared between the two groups. At the same time the two groups of P3 cells were induced for chondrogenic differentiation using pellet culture for 3 weeks in vitro. Gross observation, immunohistochemical staining of collagen Ⅱ were used to evaluate the results of each group. Based on the study mentioned above, the DMEM+bFGF culture group was subcultured to P8 to observe the morphological changes of each subculture. After chondrogenic differentiation using pellet culture for 3 weeks in vitro, gross observation and immunohistochemical staining of collagenⅡwere evaluated as before. Results The cell morphology, cell yield and cartilage differentiation in DMEM+bFGF group was far greater than that of DMEM group. Under the culture system of DMEM+bFGF, cells passaged at 1:3 could still maintain a good cell morphology in P6 generation. Immunohistochemistry showed that cartilage-specific extracellular matrix typeⅡcollagen was expressed better in P1-4 generation. Conclusion bFGF can significantly promote the proliferation andchondrogenesis of hBMSCs. P1-4 generation cells in DMEM+bFGF culture system is suitable for in vitro cartilage construction.关键词
碱性成纤维细胞生长因子/人骨髓间充质干细胞/细胞代次/软骨再生Key words
Basic fibroblast growth factor/Bone marrow stromal stem cells/Cell generation/Cartilage regeneration分类
生物科学引用本文复制引用
赵丹丹,陶然,刘豫,曹谊林,周广东..bFGF对hBMSCs增殖及成软骨能力影响的实验研究[J].组织工程与重建外科杂志,2017,13(6):318-321,4.
