安徽农业科学2017，Vol.45Issue(31)：1-3,11,4.
高效液相色谱法测定海洋生物中牛磺酸的含量
Detection of Taurine in Marine Life by High Performance Liquid Chromatography
摘要
Abstract
[Objective] The aim was to establish the analytical method of taurine by high performance liquid chromatograph and detect the taurine content in different seafood tissues.[Method] The pre-column derivatization of o-phthalaldehyde (OPA) was used to detect the taurine by studying the effects of flow phase ratio,flow rate,ratio of derivatization agent and derivatization time.[Result]The linear range (R2 ＞ 0.99)was in the range of 10-400 μg/mL at the taurine∶ OPA =1∶ 2,derivatization time 2 min,acetonitrile∶ water =15∶ 85,flow rate 0.5 mL/min,wavelength 360 nm.The relative standard deviation of the parallel samples was 2.9％-7.4％.The content of taurine in different seafood was tested by using this method.The results showed that taurine was determined in all selected marine shellfish,crustaceans and fish.The highest content of taurine was observed in the baby clam,which was up to 11.2 mg/g.Compared with different tissues of fish,the content of taurine in muscle was the higher,while the lower in gill.[Conclusion] The results of this study enrich the data of taurine content in marine life,and provide reference for the further study of the synthesis mechanism of taurine in different tissues.关键词
海产品/高效液相色谱/牛磺酸Key words
Seafood/High performance liquid chromatography/Taurine分类
农业科技引用本文复制引用
柳小英,毛丽沙,鲍敏洁,周瑾,金火喜..高效液相色谱法测定海洋生物中牛磺酸的含量[J].安徽农业科学,2017,45(31):1-3,11,4.基金项目
浙江海洋大学人才引进科研启动项目(Q1420) （Q1420）
2016年国家级大学生创新创业训练计划项目(201610340005) （201610340005）
2016年舟山市科技局浙江海洋大学专项(2016C41002). （2016C41002）
