安徽农业科学2017，Vol.45Issue(32)：6-8,3.
石蜡切片方法的改良
Method Improvement of Paraffin Section
赵亚林 1闫青地 1冯佳佳 1肖锋 1杨卓 1王凤茹 1董金皋1
作者信息
- 1. 河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室,河北保定071001
- 折叠
摘要
Abstract
[Objective] The aim was to make paraffin section safely and fast.[Method] Different methods were used to transparent and dewax the plant material and embedding,and the material was made into a transparent glass slide,and the n-butanol could instead of xylene.The other steps were the same as the traditional paraffin section method.[Result] The n-butanol not only could be mixed with ethanol,but also could be mixed with neutral gum.The n-butanol was relatively gentle,could obtain transparent tissue after formation,had no obvious shrinkage,deformation and transparent problem of long time,and reduced the damage to experimental staff.[Conclusion] The method could make paraffin section safely and fast.关键词
石蜡切片/透明剂/正丁醇/花Key words
Paraffin section/Clearing agent/n-butanol/Flower分类
农业科技引用本文复制引用
赵亚林,闫青地,冯佳佳,肖锋,杨卓,王凤茹,董金皋..石蜡切片方法的改良[J].安徽农业科学,2017,45(32):6-8,3.
