北京林业大学学报2017，Vol.39Issue(11)：75-81,7.DOI:10.13332/j.1000--1522.20170214
3种植物次生代谢物质对舞毒蛾的杀虫活性分析
Insecticidal activity analysis of three plant secondary metabolites on Lymantria dispar
摘要
Abstract
In order to investigate the insecticidal activity and mechanism of matrine, oxymatrine and kaempferol on 3rd instar larvae of Lymantria dispar, the effects of three plant secondary metabolites on the survival and three enzymes ( GST, AChE and CarE) of L. dispar were analyzed by bioassay, in vitro and in vivo enzyme activity assay. The results indicated that matrine, oxymatrine and kaempferol all possessed a highly insecticidal activity on 3rd instar larvae of L. dispar, and matrine showed the highest insecticidal activity among three plant secondary metabolites with a LC50 value of 0. 420 mg/mL. In vitro enzyme activity assays showed that the activities of GST, AChE and CarE could be inhibited by three plant secondary metabolites. The IC50 values of oxymatrine against GST and AChE were 0. 928 and 0. 717 mg/mL, respectively, while the IC50 value of matrine against CarE was 0. 436 mg/mL, which was the highest inhibition. Furthermore, the results of in vivo enzyme activity assay indicated that matrine had the highest inhibition on GST, which showed the lowest activity at 48 hours after treatment and was 39% of the control at the same stage. Moreover, oxymatrine had the highest inhibition on AChE and CarE, and their activities were 44% and 40% of the control at 72 hours after treatment, respectively. The above results indicate that the inhibition of matrine, oxymatrine and kaempferol on three enzymes ( GST, AChE and CarE) of L. dispar is one of the reasons for their insecticidal activity.关键词
苦参碱/氧化苦参碱/山奈酚/舞毒蛾/解毒酶Key words
matrine/oxymatrine/kaempferol/Lymantria dispar/detoxifying enzyme分类
农业科技引用本文复制引用
王亚军,邹传山,王若茜,林连男,张国财..3种植物次生代谢物质对舞毒蛾的杀虫活性分析[J].北京林业大学学报,2017,39(11):75-81,7.基金项目
中央高校基本科研业务费专项(2572014CA15)、哈尔滨市应用技术研究与开发项目(2016RAXXJ035)、黑龙江省森工总局科研项目(sgzjT2015005). （2572014CA15）
