大连工业大学学报2017，Vol.36Issue(6)：406-410,5.
紫外线照射对刺参体腔细胞吞噬活性的影响
Effect of UVA on the phagocytic activity of coelmocyte in sea cucumber Stichopus japonicus
摘要
Abstract
The coelmocyte phagocytic activity of S. japonicus was measured with flow cytometry (FCM ) and laser scanning confocal microscope (LSCM ).Phagocytizing rate (PR) of coelmocyte in suspensions with different fluorescent microsphere concentrations in S.japonicus was tested in vivo and in vitro.Fluorescent microspheres could not be detected by LSCM in vivo ,while the induction of phagocytosis in vitro via injection was feasible. The results showed that the volume ratio of fluorescent microspheres to coelomic cells of 1:1500 was a suitable proportion for PR evaluation of coelmocyte in S. japonicus.According to FCM test ,PR of coelmocyte reached to the maximum after 1 h and then gradually declined ,which demonstrated consistent results with LSCM.Meanwhile ,the mortality rate (MR) of coelomocyte in S. japonicus continued rising throughout the trial.It was concluded that UVA irradiation could cause a certain degree of damage to the immune function of coelmocyte ,its MR was possibly associated with PR in this study.The freshness of S. japonicas could be determined by detecting the change of PR from S. japonicas coelmocyte.关键词
刺参/体腔细胞/吞噬/紫外线/死亡率Key words
Stichopus japonicus/coelmocyte/phagocytic/UVA/mortality分类
轻工纺织引用本文复制引用
田景玉,丁月,张晶,姜鹏飞,周大勇,宋亮..紫外线照射对刺参体腔细胞吞噬活性的影响[J].大连工业大学学报,2017,36(6):406-410,5.基金项目
国家自然科学基金项目(31401562) （31401562）
辽宁省农业领域青年科技创新人才培养计划项目(2015002) （2015002）
辽宁省教育厅科学研究一般项目(L2014220). （L2014220）
