大连工业大学学报2017，Vol.36Issue(6)：432-435,4.
大孔吸附树脂分离纯化2′-脱氧腺苷
Separation and purification of 2′-deoxyadenosine by macroporous adsorption resin
吴明杰 1杜杰 1崔励1
作者信息
- 1. 大连工业大学 轻工与化工学院 ,辽宁 大连 116034
- 折叠
摘要
Abstract
The technique of separation and purification of 2′-deoxyadenosine by macroporous adsorption resin was systematically investigated.The adsorption capacity of seven different kinds of resins were studied based on the static adsorption. The results showed that D101 resin was the optimal sorbent and the procedure of desorption were sequential filtration with 0.1 mol/L Na2 CO3 solution and 30% ethanol at pH 8.0.The optimum operating parameters were determined by dynamic adsorption as follows :sample flow rate of 2.0 mL/min ,elution flow rate of 1.5 mL/min ,eluting with 4.5 times column volume of 0.1 mol/L Na2 CO3 ,3 times column volume of 30% ethanol orderly. Under the condition ,the content of 2-deoxyadenosine is as high as 78.7% ,and the yield could reach t o 93.9%.关键词
脱氧腺苷/大孔吸附树脂/纯化Key words
deoxyadenosine/macroporous adsorption resin/purification分类
化学化工引用本文复制引用
吴明杰,杜杰,崔励..大孔吸附树脂分离纯化2′-脱氧腺苷[J].大连工业大学学报,2017,36(6):432-435,4.
