发光学报2017，Vol.38Issue(12)：1561-1566,6.DOI:10.3788/fgxb20173812.1561
谷胱甘肽比率荧光探针分子的设计合成及其光谱性能
Synthesis of Ratiometric Fluorescent Probe for The Recognition of Glutathione
摘要
Abstract
Two fluorescent groups of quinoline and dansylamide were introduced into ligand L1 to-gether, and a trinuclear zinc metal-organic compound H-1 as a ratiometric fluorescent probe was self-assembled by L1 and zinc ion, and the sensitive recognition of γ-glutamyl-cysteinyl-glycine ( GSH) was realized by H-1. The recognition process was studied by UV-Vis, fluorescence spectra, 1 H NMR and ESI-MS. UV-Vis adsorption titration results show that the addition of GSH to the solu-tion of H-1 causes a significant absorbance decrease at 425 nm and an obvious absorbance increase at 320 nm. The isobestic point is 355 nm. The equilibrium constant lgK of 4. 03 ± 0. 11 is obtained with the absorbance value at 320 nm. This indicates that 1:1 stoichiometric host-guest complexation of H-1 for GSH is formed. The fluorescence spectra show that the addition of GSH to the solution of H-1 causes an obvious dansyl-based fluorescence decrease at 513 nm upon excitation at 340 nm with a significant red-shift, and an increase of quinoline-based fluorescence at 396 nm. The ratio of dan-syl-based fluorescence to quinoline-based fluorescence can be used to detect GSH and the detection limit is up to 2 . 5 × 10 -6 mol?L-1 .关键词
谷胱甘肽/比率荧光探针/三核锌大环化合物/识别Key words
glutathione ( GSH)/ratiometric fluorescent probe/trinuclear zinc metal-organic compound/recognition分类
数理科学引用本文复制引用
吴红梅,郭宇..谷胱甘肽比率荧光探针分子的设计合成及其光谱性能[J].发光学报,2017,38(12):1561-1566,6.基金项目
国家自然科学基金(21601075) （21601075）
辽宁省自然科学基金(2015020249)资助项目Supported by National Natural Science Foundation of China ( 21601075 ) （2015020249）
Natural Science Foundation of Liaoning Province (2015020249) （2015020249）
