发光学报2017，Vol.38Issue(12)：1567-1574,8.DOI:10.3788/fgxb20173812.1567
氮掺杂高量子产率荧光碳点的制备及其体外生物成像研究
Preparation of N-doped Fluorescent Carbon Dots with High Quantum Yield for In-vitro Bioimaging
摘要
Abstract
In order to obtain carbon dots with high quantum yield, a simple two-step solvothermal method was used to prepare fluorescent N-doped carbon dots with citric acid as carbon source and three isomers of phenylenediamine as nitrogen source. The carbon dots were characterized by Trans-mission electron microscopy ( TEM) , ultraviolet-visible spectrophotometry ( UV-Vis) , fluorescence spectrophotometry, Fourier transform infrared spectra ( FTIR ) , X-ray photoelectron spectroscopy ( XPS) , followed by studying the cytotoxicity and in-vitro bioimaging. The results show that three kinds of high quantum yield carbon dots ( Y=52%, 60 . 4% and 53 . 2%) possessing uniform size and excellent dispersibility have been successfully prepared, and the average size is 4. 5, 5. 3, 5. 2 nm. The prepared carbon dots with hydroxyl, carboxyl, amine and other groups on the surface can emit bright blue fluorescence under the excitation of ultraviolet light, holding favorable optical stabil-ity at the same time. In addition, the cell imaging experiments indicate that the three kinds of carbon dots have good biocompatibility, capable of rapidly entering cells and successfully applied to fluorescence imaging of cells.关键词
碳点/高量子产率/体外生物成像Key words
carbon dots/high quantum yield/in-vitro bioimaging分类
数理科学引用本文复制引用
姜杰,李士浩,严一楠,何丹农..氮掺杂高量子产率荧光碳点的制备及其体外生物成像研究[J].发光学报,2017,38(12):1567-1574,8.基金项目
上海市浦江人才项目( 15PJ1432200 ) （ 15PJ1432200 ）
国家重点研发计划( 2016YFA0201204 ) （ 2016YFA0201204 ）
上海市科学技术委员会项目(174419048000) （174419048000）
上海市青年科技启明星项目(17QB1402900)资助Supported by Pujiang Talent Project of Shanghai (15PJ1432200) （17QB1402900）
National Key Research and Development Plan (2016YFA0201204) （2016YFA0201204）
Project of Shanghai Municipal Science and Technology Committee (174419048000) （174419048000）
Youth Science and Technology Star Project of Shanghai (17QB1402900) （17QB1402900）
