福建农林大学学报（自然科学版）2017，Vol.46Issue(6)：708-715,8.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.06.018
响应面分析法优化莲子心多糖的提取工艺
Optimization of extraction technology of polysaccharides from lotus plumula by response surface methodology
摘要
Abstract
In order to investigate hot water extraction technology for extracting polysaccharides from lotus plumula, effects of extraction temperature, time, ratio of water volumn to raw material weight ( liquid/material ratio) on the yield of polysaccharides were evaluated by response surface methodology. Moreover, microstructure of lotus powder before or after extraction were examined by environmental scanning electron microscope and lotus plumula was detected by infrared spectrum scanning. The results showed that the optimum extraction condition was to extract lotus plumula at 92 ℃ for 2.6 h under liquid/material ratio of 50 : 1 ( mL : g) . Under this condition, the yield of polysaccharides was up to (14.49±0.06)%, which was consistent with the predicted theoretical value. Environmental scanning electron microscope showed that lotus powder was very different from it before extraction. Also polysaccha-ride molecules aggregated and formed a porous network structure along with the extraction. Infrared spectrum scanning showed that polysaccharide of lotus seed was mainly composed of furan ring and alpha glycosidic bond, proving that polysaccharide was fully re-leased from cell membrane after a long period of water immersion and thermal effect. Furthermore, response surface methodology was a potential method for optimizing extraction technology of lotus plumula.关键词
莲子心/多糖/响应面分析法/提取工艺Key words
lotus plumula/polysaccharides/response surface methodology/extraction technology分类
轻工纺织引用本文复制引用
陈培琳,陈钏杰,周雨嘉,沈瑾烨,张怡,郑宝东,曾红亮..响应面分析法优化莲子心多糖的提取工艺[J].福建农林大学学报（自然科学版）,2017,46(6):708-715,8.基金项目
福建省科技创新领军人才项目(KRC16002A) （KRC16002A）
福建省高等学校科技创新团队支持计划项目(闽教科[2012]03号) （闽教科[2012]03号）
福建农林大学杰出青年科研人才计划项目( XJQ201714) （ XJQ201714）
福建农林大学发展基金项目( KFA17197A) . （ KFA17197A）
