福建农业学报2017，Vol.32Issue(8)：813-817,5.DOI:10.19303/j.issn.1008-0384.2017.08.001
半番鸭源鸭1型甲肝病毒亚型的分离鉴定及其VP1基因分析
Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings
摘要
Abstract
A strain of duck hepatitis A virus 1 subtype a (DHAV-1a),coded as FJ1605,was isolated from the mule ducklings with pancreatitis.It was identified by RT-PCR analysis and a neutralization test in duck embryos that showed the strain specifically being neutralized by the anti-DHAV-1a hyperimmune serum.Molecular characteristics of VP1 gene of the virus was analyzed to indicate the nucleotide to be 714 bp in length with a homology with those of other DHAV-1a isolates ranging from 98.1％ to 99.7 ％ and sharing the greatest similarity to strain FJ1220.On the other hand,the homology between FJ1605 and DHAV-2 was merely 65.7％;and,that between FJ1605 and DHAV-3,approximately 68.3％.Phylogenetic analysis on the VP1 gene also showed FJ1605 to be in the viral lineage of DHAV-1a.Furthermore,an animal infection test clearly displayed the same clinical symptoms as those shown inclinical cases.Thus,it was concluded that the pancreatitis in the mule ducklings was caused by DHAV-1a.关键词
鸭1型甲肝病毒亚型/半番鸭源/VP1基因Key words
DHAV-1a/mule duckling/VP1 gene分类
农业科技引用本文复制引用
傅秋玲,黄瑜,傅光华,陈翠腾,程龙飞,万春和,施少华,陈红梅,陈珍,朱春华..半番鸭源鸭1型甲肝病毒亚型的分离鉴定及其VP1基因分析[J].福建农业学报,2017,32(8):813-817,5.基金项目
现代农业产业体系建设专项(CARS-43) （CARS-43）
国家自然科学基金项目(3147222) （3147222）
福建省畜禽疫病防控技术重大研发平台(2014N2003) （2014N2003）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1023-3、2016R1102) （2015R1023-3、2016R1102）
福建省自然科学基金项目(2017J01058) （2017J01058）
福建省农业科学院畜牧兽医研究所基金(MYQJ2015-6) （MYQJ2015-6）
