福建农业学报2017，Vol.32Issue(8)：828-832,5.DOI:10.19303/j.issn.1008-0384.2017.08.004
检测猪源牛病毒性腹泻病毒荧光RT-PCR方法的建立与监测分析
Establishment and Monitoring Analysis of Fluorescence RT-PCR for Detection of Bovine Viral Diarrhea Virus in Swine
摘要
Abstract
The study was aimed at developing a quantitative real-time fluorescence reverse transcriptase PCR (qRT-PCR) assay according to the conserved gene sequence of BVDV 5'-UTR for detection of bovine viral diarrhea virus (BVDV) obtained from 359 slaughtered swine serum samples of slaughterhouses in Fuzhou Prefecture.The results showed that the BVDV positive rate of slaughtered swines was 14.5％ (52 positive samples out of 359 ones).Sequence analysis on 5'-UTR fragments of 13 BVDV positive samples showed that 5"-UTR fragments of all tested samples were closely related to VEDEVAC evolution lineages,all of which were genotype I.It was indicated that the specific qRT-PCR method established based on BVDV was suitable for detection of BVDV in swines.And the BVDV infection was common in slaughtered swines of Fuzhou Prefecture.关键词
猪源牛病毒性腹泻病毒/荧光RT-PCR/屠宰猪/病原学检测Key words
BVDV in swine/fluorescence RT-PCR/slaughtered swine/pathogenic detection.分类
农业科技引用本文复制引用
白泉阳,徐磊,傅光华,曾亮明,程龙飞,黄瑜..检测猪源牛病毒性腹泻病毒荧光RT-PCR方法的建立与监测分析[J].福建农业学报,2017,32(8):828-832,5.基金项目
福建省高校杰出青年科研人才培育计划(闽教科[2015] 54号) （闽教科[2015] 54号）
福建省畜禽疫病防控技术重大研发平台项目(2014N2003) （2014N2003）
福建省中青年教师教育科研项目计划(JA15760) （JA15760）
福州市科技计划项目(2016-G-64) （2016-G-64）
