福建农业学报2017，Vol.32Issue(8)：833-836,4.DOI:10.19303/j.issn.1008-0384.2017.08.005
鸭瘟病毒的分离与初步鉴定
Isolation and Identification of Duck Plague Virus
摘要
Abstract
Tissue samples of liver and spleen from the Muscovy ducks that were suspected to have died from the duck plague were collected for this study.Viruses were isolated from the Muscovy duck embryo fibroblasts (MDEF) for a preliminary identification using hemagglutination assay (HA),immunofluorescence analysis (IFA),qPCR,PCR product sequencing,and animal infection test.Four virus strains,DPVfj1,DPVfj2,DPVfj3 and DPVfj4,were thus identified for further investigation.Subsequently,it was found that these strains (a) would not cause red blood cell agglutination on pigeon erythrocytes;(b) were tested negative on IFA with goose parvovirus,Muscovy duck parvovirus,duck reovirus,duck paramyxovirus,and Tembusu virus;(c) showed positive on test with fluorescence RT-PCR kit;(d) had a positive result on their PCR-amplified pair of primers from the specific fragment of gJ protein gene,JQ673560,and a homologygreater than 99 ％ on the sequence of the fragment with that of the duck plague virus (DPV) gJ protein gene;(e) infected 30-day-old ducks by injection and 5-day-old ducklings by co-habitation showing exactly the same clinical symptoms and postmortem signsas in the natural cases;and,(f)were recovered from the diseased birds.The results seemed to verify the fact that DPV caused the duck plaque in natural environment,and that the isolated strains possessed the virulent nature in question.关键词
鸭瘟病毒/分离/鉴定Key words
duck plague virus/isolation/identification分类
农业科技引用本文复制引用
程晓霞,林锋强,黄梅清,肖世峰,江斌,吴南洋,陈少莺,俞伏松..鸭瘟病毒的分离与初步鉴定[J].福建农业学报,2017,32(8):833-836,4.基金项目
福建省现代农业产业技术体系建设项目(2013-2018) （2013-2018）
国家现代农业产业技术体系项目(CARS-43) （CARS-43）
福建省农业科学院院科技创新团队建设项目(2016PI-6) （2016PI-6）
