福建农业学报2017，Vol.32Issue(8)：905-908,4.DOI:10.19303/j.issn.1008-0384.2017.08.018
醋酸铅对蚕豆根尖细胞微核率的影响
Effect of Lead Acetate on Micronucleus Count in Root-tip Cells of Vicia faba
摘要
Abstract
To determine the genetic injury on the root-tip cells of Vicia faba caused by lead in water,a micronucleus assay was conducted.The genotoxic effect of lead acetate in the concentrations ranging from 0.5 mg · L-1 to 30 mg · L 1 were monitored and pollution indices calculated.The results showed that micronuclei in the cells increased with increasing lead concentration.When the cells were exposed to lead acetate at the concentration of 30.0 mg · L-1 for 6 h,the number of micronuclei in the cells were significantly higher than that in control (P＜0.05).The pollution index was greater than 3.5,which was considered as a heavily polluted condition.Moreover,in the cases where the cells were treated with lead concentrations at 1.0-30.0 mg · L-1 for 12 h,similar results were observed at an extremely significant level (P＜0.01).At a same lead concentration,the 12 h treatments affected the cells significantly greater than the 6 h treatments.However,the difference was only at a significant level (P＜0.05) for 1 mg Pb · L 1.It seemed apparent that lead was absorbed to causegenetic injury to cells in the roots of V.faba,and the toxicity increased with dosage and time of the exposure.关键词
蚕豆/醋酸铅/微核试验/微核率/污染指数Key words
Vicia faba/lead acetate/micronucleus assay/micronucleus occurrence rate/pollution index分类
医药卫生引用本文复制引用
莫金钢,宋思闻,张丽辉,于岩鹏,宋凯..醋酸铅对蚕豆根尖细胞微核率的影响[J].福建农业学报,2017,32(8):905-908,4.基金项目
吉林省教育厅“十三五”科学技术项目(JJKH20170657KJ) （JJKH20170657KJ）
长春师范大学自然科学基金项目(长师大自科合字[2016]第006号) （长师大自科合字[2016]第006号）
吉林省大学生创新创业训练计划项目(201610205080) （201610205080）
