分析化学2017，Vol.45Issue(12)：1757-1765,9.DOI:10.11895/j.issn.0253-3820.171337
基于Cell-SELEX的核酸适配体在生化分析与生物成像中的应用
Aptamers from Cell-based Selection for Bioanalysis and Bioimaging
堵玉林 1莫柳婷 1易娅莎 1邱丽萍 1谭蔚泓1
作者信息
- 1. 分子科学与生物医学实验室,化学生物传感与计量学国家重点实验室,湖南大学化学化工学院,生命科学学院,核酸适配体湖南省工程实验室,长沙410082
- 折叠
摘要
Abstract
Aptamers are single-stranded oligonucleotides ( DNA or RNA ) selected through a technology termed "Systematic evolution of ligands by exponential enrichment" ( SELEX ) . In addition to high affinity and high specificity for their target molecules, aptamers have some advantages such as low molecular weight, easy synthesis, high chemical stability, low immunogenicity, and convenient modification. Based on the Cell-SELEX technique, a panel of aptamers which can specifically recognize target cell lines has been generated. By targeting specific membrane proteins in their native state, these aptamers can identify subtle molecular differences among different cell lines, thus have attracted a broad interest in biomedical research. In this review, we summarized the development of aptamers and their use in detection, profiling and imaging of tumor cells. Also, their perspectives were discussed.关键词
核酸适配体/Cell-SELEX/生化分析/生物成像/肿瘤细胞Key words
Aptamer/Cell-SELEX/Bioanalysis/Bioimaging/Tumor cells/Review引用本文复制引用
堵玉林,莫柳婷,易娅莎,邱丽萍,谭蔚泓..基于Cell-SELEX的核酸适配体在生化分析与生物成像中的应用[J].分析化学,2017,45(12):1757-1765,9.
