分析化学2017，Vol.45Issue(12)：1909-1914,6.DOI:10.11895/j.issn.0253-3820.171216
基于Mn掺杂ZnS量子点磷光内滤效应检测β-葡萄糖醛酸酶
Phosphorescent Inner Filter Effect-based Sensing System for Determination of β-Glucuronidase Using Manganese-doped Zinc Sulfide Quantum Dots
摘要
Abstract
The key to maximize the sensitivity of inner filter effect ( IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation or emission spectra of the fluorophore. In this work, Mn-doped ZnS quantum dots ( QDs) were chosen for IFE-based detection of β-glucuronidase ( GUS) , since the excitation of QDs was perfectly overlapped with the absorption of the substrate of GUS, namely 4-nitrophenyl-β-D-glucuronide ( PNPG) . In addition, the phosphorescence emission from Mn-doped ZnS QDs could eliminate the fluorescence background from biological samples. Therefore, a simple turn-on phosphorescent GUS assay was developed, with the linear range of 10-300 U/L and limit of detection of 7 U/L (S/N=3).关键词
β-葡萄糖醛酸酶/Mn-ZnS量子点/内滤效应/大肠杆菌Key words
β-Glucuronidase/Manganese-doped zinc sulfide quantum dots/Inner-filter effect/Escherichia coli引用本文复制引用
唐丹丹,张金懿,侯贤灯,吴鹏..基于Mn掺杂ZnS量子点磷光内滤效应检测β-葡萄糖醛酸酶[J].分析化学,2017,45(12):1909-1914,6.基金项目
本文系国家自然科学基金项目(No.21475090)资助 This work was supported by the National Natural Science Foundation of China (No.21475090). （No.21475090）
