法医学杂志2017，Vol.33Issue(5)：516-521,6.DOI:10.3969/j.issn.1004-5619.2017.05.015
SiFaTM23Plex试剂盒(提取测试版)在汉族人群中的法医学调查
Forensic Investigation in Han Population by SiFaTM 23 Plex Kit (Beta Version)
摘要
Abstract
Objective To investigate the performance of SiFaTM 23 Plex Kit (beta version) and its population genetics of STR loci in Han population.Methods Genotyping was performed for 1 000 unrelated healthy Han individuals by the kit.The efficiency of the kit was tested,and the frequency data and population genetics parameter information of STR loci were analysed statistically.Results The minimum amplification system could be 6.25 μL.In 25 μL standard reaction system,a satisfied genotyping profiles could be obtained with the DNA content as low as 125 pg.Among the 1 000 individuals,267 alleles were detected by 21 autosomal STR loci of the kit,which conformed to Hardy-Weinberg equilibrium.Fifteen and eleven alleles were observed at the newly added STR loci D1S1656 and D10S1248,respectively,which showed a high polymorphism information content.Conclusion SiFaTM 23 Plex Kit (beta version) is excellent in testing blood samples.Its accuracy,repeatability and sensitivity can satisfy the need of forensic practice,which makes it be applied to forensic-related case work and DNA database establishment.关键词
法医遗传学/短串联重复序列/SiFaTM 23 Plex试剂盒(提取测试版)/汉族Key words
forensic genetics/short tandem repeat/SiFaTM 23 Plex Kit (beta version)/Han nationality分类
社会科学引用本文复制引用
巩五虎,薛少华,张岩,朱如心,赵珍敏..SiFaTM23Plex试剂盒(提取测试版)在汉族人群中的法医学调查[J].法医学杂志,2017,33(5):516-521,6.基金项目
上海市司法鉴定专业技术服务平台资助项目(16DZ2290900) （16DZ2290900）
上海市标准研制项目(14DZ0502500) （14DZ0502500）
