高等学校化学学报2017，Vol.38Issue(12)：2193-2197,5.DOI:10.7503/cjcu20170232
海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备
Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica
摘要
Abstract
The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatogra-phy, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-poly-merized sulfated fuco-oligosaccharide / fucose, and the major fractions could be expressed with the formula of Fuc1~3 S1~3 , Fuc1~2 S1~2 , Fuc1~2 S, and FucGlcAS.关键词
褐藻多糖硫酸酯/岩藻寡糖/酸水解Key words
Fucoidan/Fuco-oligosaccharide/Acid hydrolysis分类
化学化工引用本文复制引用
耿丽华,金维华,王晶,张全斌..海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备[J].高等学校化学学报,2017,38(12):2193-2197,5.基金项目
国家自然科学基金(批准号: 41506165)、 山东省重点研发计划项目(海洋医用食品) (批准号: 2016YYSP002)、 山东省自然科学基金(批准号: ZR2014DQ024)和青岛市应用基础研究计划-青年专项(批准号: 15-9-1-74-jch)资助. Supported by the National Natural Science Foundation of China(No.41506165), the Focus on Research and Development Plan in Shandong Province(Marine Medical Food), China(No.2016YYSP002), the Shandong Provincial Natural Science Foundation of China(No.ZR2014DQ024) and the Science and Technology Program of Applied Basic Research Projects of Qingdao Municipal, China(No.15-9-1-74-jch). （批准号: 41506165）
