医学分子生物学杂志2017，Vol.14Issue(6)：342-346,5.DOI:10.3870/j.issn.1672-8009.2017.06.007
人鳞状细胞癌细胞株A431中Bmi-1基因的RNAi对其增殖的影响
Effect of RNAi ofBmi-1 gene in human squamous cell carcinoma cell line A431 on its proliferation
王应权 1王晨霞 1李强 1马铭 1郭歌1
作者信息
- 1. 榆林市第二医院皮肤科 陕西省榆林市,719000
- 折叠
摘要
Abstract
Objective To investigate the effect of RNA interference(RNAi)of B-cell specific moloney murine leukemia virus insertion site1(Bmi-1)gene in human squamous cell carcinoma cell line A431 on its proliferation.Methods ]RNAi of Bmi-1 gene was transfected to squamous cell carcinoma cell line A431, and the Bmi-1-RNAi silencing vectors of A431 cell lines were screened by reverse transcription polymerase chain reaction(RT-PCR).The expression of Bmi-1 protein was determined by Western blotting, and the inhibition of cell proliferation was detected by using methyl thiazolyl tetrazolium(MTT).Cell cycle was flow cytometrically determined.Results Two days af-ter transfection with Bmi-1 shRNA, the A431 cells became small, the gap became larger, and the growth rate slowed down.The expression of Bmi-mRNA was significantly lower in Bmi-1 shRNA transfection group than in control group(P<0.05).The expression of Bmi-1 protein in the Bmi-1 shRNA transfection group was significantly lower than that in the blank control group.The percentage of S phase cells was higher and the percentage of G2/M phase cells was lower in Bmi-1 shRNA transfection group than in blank control group(P<0.05).Conclusion RNAi can down-regulate the expression of Bmi-1 mRNA and protein, inhibit the differentiation and proliferation of A431 cells and block their growth cycle.关键词
鳞状细胞癌/A431/Bmi-1基因/细胞增殖Key words
Squamous cell carcinoma/A431/Bmi-1 gene/Cell proliferation分类
医药卫生引用本文复制引用
王应权,王晨霞,李强,马铭,郭歌..人鳞状细胞癌细胞株A431中Bmi-1基因的RNAi对其增殖的影响[J].医学分子生物学杂志,2017,14(6):342-346,5.
