海南医学院学报2017，Vol.23Issue(18)：2484-2487,4.DOI:10.13210/j.cnki.jhmu.20170920.010
肠道菌群检测对溃疡性结肠炎患者肠黏膜内炎症反应、免疫应答的评估价值
Evaluation value of intestinal flora detection for intestinal mucosal inflammation and immune response in patients with ulcerative colitis
摘要
Abstract
Objective:To study the evaluation value of intestinal flora detection for intestinal mucosal inflammatory response and immune response in patients with ulcerative colitis.Methods:The patients who were diagnosed with ulcerative colitis in Zigong Fifth People's Hospital between March 2015 and February 2017 were selected as the UC group,and those who were diagnosed with colonic polyps were selected as the control group.Fresh excreta were collected to detect the number of intestinal flora,and the diseased intestinal mucosa tissue was collected to detect the expression of inflammatory response molecules and immune cell transcription factors.Results:enterococcus contents in intestinal tract and TLR4,NF-kB,TNF-α,HMGB-1,T-bet and RORC mRNA expression levels in intestinal mucosa of UC group were significantly higher than those of control group while bifidobacteria contents in intestinal tract and SOCS2,SOCS3,Foxp3 and GATA-3 mRNA expression levels were significantly lower than those of control group;TLR4,NF-kB,TNF-α,HMGB-1,T-bet and RORC mRNA expression levels in intestinal mucosa of UC patients with grade Ⅱ and grade Ⅲ flora disturbance were significantly higher than those of UC patients with normal flora and grade Ⅰ flora disturbance while SOCS2,SOCS3,Foxp3 and GATA-3 mRNA expression levels were significantly lower than those of UC patients with normal flora and grade Ⅰ flora disturbance;TLR4,NF-kB,TNF-α,HMGB-1,T-bet and RORC mRNA expression levels in intestinal mucosa of UC patients with grade Ⅲ flora disturbance were significantly higher than those of UC patients with grade Ⅱ flora disturbance while SOCS2,SOCS3,Foxp3 and GATA-3 mRNA expression levels were significantly lower than those of UC patients with grade Ⅱ flora disturbance.Conclusion:The intestinal flora disturbance in patients with ulcerative colitis can result in inflammatory response activation and immune response disorder.关键词
溃疡性结肠炎/肠道菌群紊乱/炎症反应/免疫应答Key words
Ulcerative colitis/intestinal flora disturbance/inflammatory response/immune response分类
医药卫生引用本文复制引用
邹艳,詹孔才,吴明德..肠道菌群检测对溃疡性结肠炎患者肠黏膜内炎症反应、免疫应答的评估价值[J].海南医学院学报,2017,23(18):2484-2487,4.基金项目
四川省卫生厅基金项目资助(130516) （130516）
It is supported by Fund Project of Health and Family Planning Department of Sichuan Province (130516). （130516）
