湖南农业大学学报（自然科学版）2017，Vol.43Issue(6)：597-603,7.DOI:10.13331/j.cnki.jhau.2017.06.004
巴西橡胶树HbRALF34的克隆及表达分析
Cloning and expression analysis of HbRALF34 from Hevea brasiliensis
摘要
Abstract
Based on the latex transcriptome date, a cDNA sequence of rapid alkalinization factor (RALF) family gene was identified by RT-PCR. The cDNA is 767 bp in size with a 411 bp open reading frame and encodes 136 amino acids. BLASTX searches of GenBank showed that the deduced amino acid sequences of RALF gene was similar to AtRALF34,thus was named as HbRALF34. Expression profiles of HbRALF34 in different tissues of Hevea brasiliensis clone Reyan 7-33-97 and in response to ethylene (ET), jasmonic acid (JA), wounding, hydrogen peroxide (H2O2), high salt, low temperature and drought were assayed via real-time quantitative PCR (RT-qPCR). The results suggested that HbRALF34 was ubiquitously expressed in the leaves, flowers, barks and latex of rubber tree, and its highest expression was in latex. Compared with healthy rubber tree, the expression level of HbRALF34 was increased in latex and bark of tapping panel dryness rubber tree. Moreover, the expression of HbRALF34 was induced by ET, JA, H2O2 and wounding treatments, but was inhibited by high salt stress, low temperature and drought treatments. All these results suggest that HbRALF34 is likely involved in signal transduction, regulating rubber biosynthesis and latex flow, as well as responding to stress in Hevea brasiliensis.关键词
巴西橡胶树/HbRALF34/快速碱化因子/基因克隆/表达分析Key words
Hevea brasiliensis/HbRALF34/rapid alkalinization factor/gene cloning/expression analysis分类
农业科技引用本文复制引用
聂智毅,黎瑜,康桂娟,蔡海滨,曾日中..巴西橡胶树HbRALF34的克隆及表达分析[J].湖南农业大学学报（自然科学版）,2017,43(6):597-603,7.基金项目
国家自然科学基金项目(31770642,31270713) （31770642,31270713）
海南省自然科学基金项目(20153108) （20153108）
