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水杨酸和茉莉酸甲酯对艾纳香叶片左旋龙脑与总黄酮含量的影响

官玲亮 夏奇峰 庞玉新 于福来 黄梅 陈振夏

湖南农业大学学报(自然科学版)2017,Vol.43Issue(6):610-614,5.
湖南农业大学学报(自然科学版)2017,Vol.43Issue(6):610-614,5.DOI:10.13331/j.cnki.jhau.2017.06.006

水杨酸和茉莉酸甲酯对艾纳香叶片左旋龙脑与总黄酮含量的影响

The influence of salicylic acid (SA) and methyl jasmonate (MeJA) to concentrations of L-borneol and total flavonoids in leaves of Blumea balsamifera

官玲亮 1夏奇峰 1庞玉新 2于福来 1黄梅 1陈振夏1

作者信息

  • 1. 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室/海南省艾纳香工程技术研究中心,海南 儋州 571737
  • 2. 贵州大学生命科学学院/贵州省中药材重点实验室,贵州 贵阳550025
  • 折叠

摘要

Abstract

Blumea balsamifera L. DC. was used as test material in this study. Salicylic acid (SA) and Methyl Jasmonate (MeJA) with different concentrations (10, 1, 0.1, 0.01 mmol/L of aqueous solution) were sprayed respectively to the leaves of different position, the control was sprayed with water. Then the contents of L-borneol and total flavonoids were determined to explore the response of leaves to different concentrations of SA and MeJA. The results showed that except for the treatment of 10 mmol/L MeJA, the content of L-borneol of the other treatments reached the maximum value at 72 h after the young leaves sprayed with MeJA. The content of L-borneol reached maximum value at 72 h (3.043 mg/g) after the mature leaves sprayed with 1.0 mmol/L MeJA, which was significantly higher than that of the control. Exceptfor the treatment of 0.01 mmol/L MeJA, the total flavonoids of the other treatments reached the maximum value at 48 h after the young leaves sprayed with MeJA. The total flavonoids reached maximum value at 72 h (19.246 mg/g) after the old leaves treated with 1.0 mmol/L MeJA. The L-borneol contents of the treatments of mature leaves and old leaves sprayed with 0.01 mmol/L reached 2.418 and 2.034 mg/g respectively at 24 h, which were significant higher than those of the control group. Compared with other concentrations, 0.1mmol/L SA had higher total flavonoids (young leaves 6.287 mg/g,mature leaves 8.172 mg/g and old leaves 11.827 mg/g ) at 24 h. In conclusion, 1 mmol/L MeJA could effectively promote the synthesis of L-borneol and total flavonoids and 0.01 mmol/L SA was advantageous to accumulate L-borneol.

关键词

艾纳香/水杨酸/茉莉酸甲酯/L-龙脑/总黄酮

Key words

Blumea balsamifera/salicylic acid/methyl Jasmonate/L-borneol/total flavonoids

分类

农业科技

引用本文复制引用

官玲亮,夏奇峰,庞玉新,于福来,黄梅,陈振夏..水杨酸和茉莉酸甲酯对艾纳香叶片左旋龙脑与总黄酮含量的影响[J].湖南农业大学学报(自然科学版),2017,43(6):610-614,5.

基金项目

国家自然科学基金项目(81202910) (81202910)

海南省自然科学基金项目(817263) (817263)

海南省中药现代化专项(ZY201410) (ZY201410)

湖南农业大学学报(自然科学版)

OA北大核心CSCDCSTPCD

1007-1032

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