湖南农业大学学报(自然科学版)2017,Vol.43Issue(6):610-614,5.DOI:10.13331/j.cnki.jhau.2017.06.006
水杨酸和茉莉酸甲酯对艾纳香叶片左旋龙脑与总黄酮含量的影响
The influence of salicylic acid (SA) and methyl jasmonate (MeJA) to concentrations of L-borneol and total flavonoids in leaves of Blumea balsamifera
摘要
Abstract
Blumea balsamifera L. DC. was used as test material in this study. Salicylic acid (SA) and Methyl Jasmonate (MeJA) with different concentrations (10, 1, 0.1, 0.01 mmol/L of aqueous solution) were sprayed respectively to the leaves of different position, the control was sprayed with water. Then the contents of L-borneol and total flavonoids were determined to explore the response of leaves to different concentrations of SA and MeJA. The results showed that except for the treatment of 10 mmol/L MeJA, the content of L-borneol of the other treatments reached the maximum value at 72 h after the young leaves sprayed with MeJA. The content of L-borneol reached maximum value at 72 h (3.043 mg/g) after the mature leaves sprayed with 1.0 mmol/L MeJA, which was significantly higher than that of the control. Exceptfor the treatment of 0.01 mmol/L MeJA, the total flavonoids of the other treatments reached the maximum value at 48 h after the young leaves sprayed with MeJA. The total flavonoids reached maximum value at 72 h (19.246 mg/g) after the old leaves treated with 1.0 mmol/L MeJA. The L-borneol contents of the treatments of mature leaves and old leaves sprayed with 0.01 mmol/L reached 2.418 and 2.034 mg/g respectively at 24 h, which were significant higher than those of the control group. Compared with other concentrations, 0.1mmol/L SA had higher total flavonoids (young leaves 6.287 mg/g,mature leaves 8.172 mg/g and old leaves 11.827 mg/g ) at 24 h. In conclusion, 1 mmol/L MeJA could effectively promote the synthesis of L-borneol and total flavonoids and 0.01 mmol/L SA was advantageous to accumulate L-borneol.关键词
艾纳香/水杨酸/茉莉酸甲酯/L-龙脑/总黄酮Key words
Blumea balsamifera/salicylic acid/methyl Jasmonate/L-borneol/total flavonoids分类
农业科技引用本文复制引用
官玲亮,夏奇峰,庞玉新,于福来,黄梅,陈振夏..水杨酸和茉莉酸甲酯对艾纳香叶片左旋龙脑与总黄酮含量的影响[J].湖南农业大学学报(自然科学版),2017,43(6):610-614,5.基金项目
国家自然科学基金项目(81202910) (81202910)
海南省自然科学基金项目(817263) (817263)
海南省中药现代化专项(ZY201410) (ZY201410)