化学工程2017，Vol.45Issue(10)：6-11,6.DOI:10.3969/j.issn.1005-9954.2017.10.002
相分离联合层析去除类人胶原蛋白中的内毒素
Endotoxin removal from human-like collagen using Triton X-114 two-phase extraction and affinity chromatography resin
摘要
Abstract
Triton X-114 two-phase extraction was applied as the first step to remove the large amounts of endotoxins from the preparation,and affinity chromatography resin,attached with modified polylysine,was used as the second step to achieve further purification.Furthermore,the processed protein was tested in physical and chemical properties.The results show that in the Triton X-114 two-phase extraction process,the content of HLC is 2.06 mg/ mL and the sample (pH 7) is incubated at 44.71 ℃ for 3 h.Under this circumstance,endotoxin level in HLC is declined from 10 000-25 000 EU/mg to 1.5-2.0 EU/mg with high protein recovery (95.6％).Accompanied with affinity chromatography resin,endotoxin level in HLC is declined from 1.5-2.0 EU/mg to 0.025-0.25 EU/mg with 81.9％ protein recovery.These two procedures do not change the molecular mass or chemical structure of HLC.The process is simple and convenient to carry out and can reduce the production cost significantly.关键词
类人胶原蛋白/内毒素/Triton X-114双水相分离/亲和层析Key words
human-like collagen/endotoxin/triton X-114 two-phase extraction/affinity chromatography分类
化学化工引用本文复制引用
段志广,朱晨辉,张婧,范代娣..相分离联合层析去除类人胶原蛋白中的内毒素[J].化学工程,2017,45(10):6-11,6.基金项目
西安市教育厅专项基金(12JK0449) （12JK0449）
