江西农业大学学报2017，Vol.39Issue(6)：1089-1095,7.DOI:10.13836/j.jjau.2017141
黄连属植物DNA条形码研究
DNA Barcoding Evaluation in the Genus Coptis Salisb.
摘要
Abstract
The present study made a global samling of Coptis and selected four frequently used DNA bar-codes ( trnH-psbA and ycf1 from cpDNA,ITS and ETS from nrDNA) ,to evaluate their species discrimination a-bility in the genus.Based on the comprehensive analyses of the sequence characteristics,intra/inter-specific ge-netic distance,DNA barcoding gap and neighbor-joining trees,the results show that the intra-specific genetic distance in each barcode was lower than inter-specific genetic distance,no obvious "barcoding gap" was ob-served in every barcode,and species identification efficience of each barcode varied from 47% to 63%.Among the barcode combinations,the ITS+ycf1 has the same resolution as combining three or four barcodes,and the i-dentification efficience is up to 79%,indicating that the ITS+ycf1 barcode combination would be valuable to be selected as the standard DNA barcode for Coptis species identification.关键词
DNA条形码/分子鉴定/中药/黄连属Key words
DNA barcoding/molecular identification/traditional Chinese herbs/Coptis分类
农业科技引用本文复制引用
李波,刘俊,闵道长,何小慧,李波超,朱昌兰,张志勇,袁庆军,吴丁..黄连属植物DNA条形码研究[J].江西农业大学学报,2017,39(6):1089-1095,7.基金项目
国家自然科学基金项目(31360046)和国家级大学生创新创业训练计划项目(201510410006) Project supported by the National Natural Science Foundationof China (31360046) and the National University Students Innovation and Entrepreneurship Training Programs (201510410006) （31360046）
