临床神经外科杂志2017，Vol.14Issue(6)：401-405,5.DOI:10.3969/j.issn.1672-7770.2017.06.001
miR-221/miR-222家族在神经胶质瘤患者脑脊液中的表达及其诊断价值
Identification of miR-221/miR-222 in cerebrospinal fluid as biomarker for diagnosis of glioma
摘要
Abstract
Objective To explore different expression of miR-221/miR-222 in cerebrospinal fluid ( CSF) between glioma patients and controls,and investigate the potential role of CSF miR-221/miR-222 in detecting glioma.Methods A total of 31 glioma patients and 33 non-glioma patients ( controls) were enrolled in our study.Expression levels of CSF miR-221/miR-222 were analyzed by quantitative reverse transcription polymerase chain reaction ( qRT-PCR).Diagnostic value of CSF miR-221/miR-222 was assessed by receiver operating characteristic ( ROC ) method.Results CSF miR-221 and miR-222 were significantly higher in glioma patients compared with controls (all P <0.001).The expression of miR-221/miR-222 in the CSF of patients with high grade glioma was higher than that in low grade glioma,and the difference of miR-222 expression was statistically significant (1.49 fold,P =0.007).The area under the ROC curve ( AUC ) of the combination of the two miRNAs was 0.849,with sensitivity of 81.8% and specificity of 83.9%.Conclusion miR-221/miR-222 in CSF can serve as a biomarker in diagnosing glioma.关键词
胶质瘤/脑脊液/miR-221/miR-222/诊断Key words
glioma/cerebrospinal fluid/miR-221/miR-222/diagnosis分类
医药卫生引用本文复制引用
张青,仇诚,夏东彦,李英斌..miR-221/miR-222家族在神经胶质瘤患者脑脊液中的表达及其诊断价值[J].临床神经外科杂志,2017,14(6):401-405,5.基金项目
南京市科技计划项目基金(201303042) （201303042）
