临床输血与检验2017，Vol.19Issue(6)：623-627,5.DOI:10.3969/j.issn.1671-2587.2017.06.028
黑龙江省汉族人群血小板抗原1～17系统基因多态性研究
Genetic Polymorphism of HPA1-17 Alloantigen System in Heilongjiang Han Population
摘要
Abstract
Objective To analyze the allele frequencies and the polymorphisms of Human platlet antigen 1 to 17 system in Heilongjiang Han population for determination of their clinical significance. Method PCR-SSP was used for HPA1-17 genotyping among 123 unrelated Han nationality volunteer donors. The genotypes and gene frequencies of the each HPA gene system were calculated,and the difference with other populations were compared. Result The gene frequencies of HPA-1a,2a,3a,5a,6a,15a were 0.9878,0.9594 ,0.6828,0.9715,0.9837,and 0.4796,respectively,and the gene frequencies for all of HPA-4a,7a~14a,16a,17a were found to be 1.0. The gene frequencies of HPA-1b,2b,3b, 5b,6b,15b were 0.0122,0.0407,0.3171,0.0285,0.0163,and 0.5204,respectively. HPA-4b and 7b to 14b and 16b to 17b were undetactabl. The study is the first time to investigate the assortment of HPA genes and their frequencies. The 24 assortment systems were found in Heilongjiang Han population,and only 3 of HPA gene frequencies more than 10%, and 21 less than 7.5% were noted. Conclusion Distribution of the allele polymorphisms of HPA 1-17 in Heilongjiang Han population was featured compared with other regions and countries. The system of HPA-3,15 in Han population with high polymorphism in that the incompatibility rates of donor recipient HPA-3,15 were 33.93% and 37.46% in random platelets infusion.关键词
人类血小板同种抗原/基因型/多态性Key words
Human platelet antigens/Genotype/Polymorphism分类
医药卫生引用本文复制引用
颜廷宇,李鑫,丁镌,毕冬梅,王鑫,刘颖..黑龙江省汉族人群血小板抗原1～17系统基因多态性研究[J].临床输血与检验,2017,19(6):623-627,5.基金项目
本课题受2012年度黑龙江省卫生厅科技计划课题(No.2012-081)资助 （No.2012-081）
