环境与职业医学2017，Vol.34Issue(11)：964-968,5.DOI:10.13213/j.cnki.jeom.2017.17369
光气急性染毒大鼠的血液流变学变化
Changes in hemorheology of rats with acute phosgene exposure
摘要
Abstract
[Objective] To investigate the effects of phosgene exposure on hemorheological in dicators of rats.[Methods] Health adult male SD rats were randomized into a control group (n=30) and a phosgene exposure group (n=30).Blood samples of rats were collected at 1,3,6,12,and 24 h after'5-min air or phosgene (8.33 mg/L) inhalation and after intraperitoneal injection with 20％ urethane anesthesia.Routine blood test,blood gas analysis,and hemorheological indicator detection were performed.[Results] The levels of arterial oxygen partial pressure and oxygenation index decreased at 3 h after phosgene inhalation and reached bottom at 6 h,being 58.67 and 202.30 mmHg (1 mmHg=0.133 kPa),respectively.The arterial oxygen partial pressure at 6 h reached the clinical standard of respiratory failure (＜60 mmHg);the oxygenation index at 6 h reached the diagnostic criteria for acute lung injury (＜300 mmHg) and was close to the diagnostic criteria for acute respiratory distress syndrome (＜200 mmHg).Compared with the control group,the exposure group showed elevated partial pressure of carbon dioxide at each designed time point,and the trend of pH was aligned with oxygen partial pressure and oxygenation index.The red blood cell counts at 1,3,and 24h were lower in the exposure group than in the control group (P＜0.01 or P＜0.05),except 6 and 12h.Packed cell volume was increased in the exposure group and showed statistical differences at 6 and 12h compared with the control group (P＜0.01).Platelet count was decreased in the exposure group at all time points (P＜0.05).In addition,the rats administered with phosgene showed higher whole blood viscosity than the control group at varied shear rates,especially at 6h,and except the shear rate of 10s-1,the whole blood viscosity at other shear rates showed statistical differences between the two groups (P＜0.01).The plasma viscosity of the exposure group was increased at 1 h,then decreased,and no difference was found at 24h compared with the control group.The maximum erythrocyte deformability index of the exposure group was obviously decreased from 1 h,reached bottom at 12,but rebounded slightly at 24h.[Conclusion] Phosgene can induce acute lung injury and significant changes in hemorheological indicators in rats.关键词
光气/中毒/急性肺损伤/血液流变学Key words
phosgene/intoxication/acute lung injury/hemorheology分类
医药卫生引用本文复制引用
赵赞梅,郑亦沐,张雁林,李树强,赵海燕..光气急性染毒大鼠的血液流变学变化[J].环境与职业医学,2017,34(11):964-968,5.基金项目
国家自然科学基金青年科学基金项目(编号:81402641) （编号:81402641）
