环境与职业医学2017，Vol.34Issue(11)：995-998,4.DOI:10.13213/j.cnki.jeom.2017.17294
不同油脂对小鼠肠道菌群的影响
Effects of different oils on intestinal microbiota of mice
摘要
Abstract
[Objective] To study the effects of different oils on intestinal microbiota.[Methods] C57BL/6 male mice (6 weeks old,16-18g) were divided into 7 groups with 12 mice in each group.The control group was fed with an ordinary diet with a 10％ fat-energy ratio,and the other groups were fed with a high-fat diet (D12451) added with lard,soybean oil,24 degrees palm oil,33 degrees palm oil,mixture oil (soybean oil and palm oil with a mass ratio of 0.9 ∶ 1),and olive oil,respectively.After a 12-week diet intervention,blood samples were collected from the orbit of mice to detect total cholesterol and triglyceride.Residual waste was removed from colon to conduct 16S rRNA amplicon sequencing for cluster analysis and speciation analysis.MetaStat was used to analyze species abundance,obtain P value and q value (adjusted P value),and screen species with significant differences.[Results] The total cholesterol and triglyceride of the experimental groups were higher than those of the control group.The mixed oil group showed a higher level of relative abundance of Actinobacteria than the control group (q＜0.05).The lard group and the 33 degrees palm oil group showed higher levels of relative abundance of Verrucomicrobia than the control group (q ＜ 0.05).[Conclusion] A long-term high-fat diet could affect the intestinal flora of mice.Soybean oil,olive oil,and 24 degrees palm oil do not cause abnormalities in intestinal microbiota.关键词
油脂/C57BL/6/肠道菌群/血脂/放线菌/疣微菌/16S rRNAKey words
oil/C57BL/6/intestinal flora/blood fat/Actinobacteria/Verrucomicrobia/16S rRNA分类
医药卫生引用本文复制引用
朱航榉,王锋,杨贤,王兆丹,夏慧,成晨,孙桂菊..不同油脂对小鼠肠道菌群的影响[J].环境与职业医学,2017,34(11):995-998,4.基金项目
江苏省普通高校研究生科研创新计划资助项目(编号:SJZZ15_0022) （编号:SJZZ15_0022）
国家重点研发计划(编号:2016YFD0400604) （编号:2016YFD0400604）
