棉纺织技术2017，Vol.45Issue(12)：32-37,6.
一种快速检测纺织品中沙门氏菌的方法
A Method for the Rapid Detection of Salmonella in Textiles
摘要
Abstract
The methods for the rapid detection of salmonella in textiles were discussed .The rapid detection method for salmonella in textiles was established by applying loop-mediated isothermal amplification technology and combining with matrix-assisted laser desorption Ionization time-of-flight mass spectrometry technology .The invA gene order for salmonella had the specificity of pathogenic bacterial strain category .A set of salmonella LAMP primers were designed according to its properties .When the amplification effect of LAMP was suspicious or positive ,the strain was separated .Through MALDI TOF MS technology ,the results were rapidly identified . The classic referred strain was chosen for specificity test .The salmonella bacteria solutions were diluted into dif-ferent gradients for sensitivity test .The test results show that the method has better specificity and sensitivity . The test limitation can reach 4 .5 CFU/mL .It is considered that the method can be used for rapid detection of sal-monella in textiles .关键词
纺织品/沙门氏菌/快速检测/invA基因/环介导等温扩增技术Key words
Textiles/Salmonella/Rapid Detection/invA Gene/Loop-mediated Isothermal Amplification Technology分类
轻工纺织引用本文复制引用
李轲,郭会清,郭华麟,张淑霞,王新辉..一种快速检测纺织品中沙门氏菌的方法[J].棉纺织技术,2017,45(12):32-37,6.基金项目
纺织品生物安全项目关键检测技术研究及其生物安全风险评估(162102210370) （162102210370）
