农药学学报2017，Vol.19Issue(6)：701-707,7.DOI:10.16801/j.issn.1008-7303.2017.0096
山东省辣椒上尖孢炭疽复合种对吡唑醚菌酯的敏感基线及吡唑醚菌酯增效配方筛选
Baseline sensitivity to pyraclostrobin of Colletotrichum acutatum species complex collected from chili in Shandong Province and the screening of synergistic formula
摘要
Abstract
To evaluate the sensitivity ofColletotrichum acutatum species complex to pyraclostrobin, delay the development of resistance and reduce the financial cost of controlling chili anthracnose, 175 single-spore isolates ofC. acutatum species complex were collected from main chili production regions in Shandong Province. The sensitivities ofC. acutatum species complex isolates to pyraclostrobin were determined by using mycelial growth test and spore germination test. At the same time, the joint-toxicities of pyraclostrobin combined with thiophanate-methyl, carbendazim, mancozeb, tebuconazole and fludioxonil againstC. acutatum species complex were also assessed by using mycelial growth test and spore germination test. The frequency distributions of EC50 values were unimodal curves, with the average EC50 values of (0.273 ± 0.067) and (0.014 ± 0.019) μg/mL for the inhibition of mycelial growth and spore germination, respectively. Therefore, the data could be employed as the baseline-sensitivity of C. acutatum species complex to pyraclostrobin, and it could be used for the field resistance monitoring. The mean EC50 values were different among the isolates collected from different districts. The isolates from Heze were the most sensitive to pyraclostrobin, while the isolates from Weifang were the least sensitive to pyraclostrobin. The results of joint-action test indicate that the mixtures of pyraclostrobin and thiophanate-methyl at the ratios of 1:3 and 3:1 had synergistic effect on both the mycelial growth and spore germination ofC. acutatum species complex. The most obvious synergistic effects were observed for the mixture of pyraclostrobin and thiophanate-methyl at the ratio of 1:3, with synergistic ratios of 4.82 and 3.94 for mycelial growth and spore germination tests, respectively. The results provided a theoretical basis for the long-term reasonable application of pyraclostrobin.关键词
尖孢炭疽复合种/吡唑醚菌酯/辣椒/敏感基线/联合毒力/增效Key words
Colletotrichum acutatum species complex/pyraclostrobin/chili/baseline sensitivity/joint-toxicity/synergy分类
农业科技引用本文复制引用
高杨杨,禾丽菲,李北兴,林琎,慕卫,刘峰..山东省辣椒上尖孢炭疽复合种对吡唑醚菌酯的敏感基线及吡唑醚菌酯增效配方筛选[J].农药学学报,2017,19(6):701-707,7.基金项目
国家重点研发计划(2016YFD0200500) （2016YFD0200500）
国家自然科学基金(31772203). （31772203）
