农药学学报2017，Vol.19Issue(6)：708-715,8.DOI:10.16801/j.issn.1008-7303.2017.0097
胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价
Identification and preliminary evaluation of a biocontrol actinomycete strain gz-8 against Colletotrichum gloeosporioides
摘要
Abstract
An actinomycete strain gz-8 was isolated from Linyi, Shandong Province, which had a strong antagonistic effect againstColletotrichum gloeosporioides with the inhibition rate of 72.5%. Strain gz-8 was identified asStreptomyces badius based on the morphology, physiological and biochemical characteristics, combined with 16S rDNA sequence analysis. Strain gz-8 has a broad antifungal spectrum, with strong inhibitory effects on six plant pathogenic fungi. The inhibitory activity of the crude extract from strain gz-8 was evaluated. The fermentation broth extract exhibits obvious inhibitory effects on the mycelial growth (EC50=3.18 μg/mL) and conidial germination (IC50=1.92 μg/mL) ofC. gloeosporioides, which had higher activity compared to the mycelium extract of strain gz-8. The fermentation broth extract had good thermostability and pH stability. The control effect of 1000 mg/L fermentation broth extract to mango fruits infected withC. gloeosporioides was 35.94%, which had no significant difference with that of chlorothalonil at the same concentration(34.11%).关键词
胶孢炭疽菌/放线菌/栗褐链霉菌/炭疽病/菌株鉴定/生物防治Key words
Colletotrichum gloeosporioides/anthracnose/actinomycete strain/Streptomyces badius/strain identification/biological control分类
农业科技引用本文复制引用
张凯,吴曼莉,辜柳霜,郑钧月,柳志强,李晓宇..胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价[J].农药学学报,2017,19(6):708-715,8.基金项目
海南省重点研发计划(ZDYF2016155) （ZDYF2016155）
国家自然科学基金(31560045). （31560045）
