农药学学报2017，Vol.19Issue(6)：735-743,9.DOI:10.16801/j.issn.1008-7303.2017.0102
优化的QuEChERS-超高效液相色谱-串联质谱法检测黄瓜中29种杀菌剂残留
Determination of 29 fungicide residues in cucumber by modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A multiresidue method was developed for the simultaneous determination of 29 fungicides in cucumber by using modified QuEChERS-ultra performance liquid chromatography-tandem mass spetrometry (UPLC-MS/MS). The cucumber samples were first extracted by acetonitrile and the preprocessing method was optimized. TheN-(n-propyl) ethylenediamine (PSA) and graphitized carbon blacks (GCB) were used as adsorbent materials in the dispersive solid phase extraction (dSPE) process. The extraction was carried out, and the samples were detected by UPLC-MS/MS. The results demonstrate the concentrations of 29 fungicides have a good linear relationship with the corresponding peak area in the range of 0.0001-0.05 mg/kg at 0.01, 0.05 and 0.5 mg/kg spiked levels. The correlation coefficients of all fungicides are above 0.996.The average recoveries of these pesticides rang from 74% to 112%, with RSDs rang from 1.1%-17% (n=5). The LOQs (limit of quantification) are 0.0001-0.01 mg/kg. This method has been successfully applied in real samples. This method is simple, rapid, robust, efficient and inexpensive. It is suitable for the simultaneous determination of 29 fungicides in cucumber.关键词
QuEChERS/超高效液相色谱-串联质谱/黄瓜/杀菌剂/残留Key words
QuEChERS/ultra performance liquid chromatography-tandem mass spetrometry (UPLC-MS/MS)/cucumber/fungicide/residue分类
农业科技引用本文复制引用
蒋建功,杨松,林琎,周秀玲,孙丰收,慕卫..优化的QuEChERS-超高效液相色谱-串联质谱法检测黄瓜中29种杀菌剂残留[J].农药学学报,2017,19(6):735-743,9.基金项目
国家重点研发计划(2016YFD0200500) （2016YFD0200500）
国家自然科学基金(31572040). （31572040）
