农药学学报2017，Vol.19Issue(6)：755-759,5.DOI:10.16801/j.issn.1008-7303.2017.0100
抗联苯菊酯枸杞蚜虫羧酸酯酶活性及4种常用杀虫剂对酶的抑制作用
Activity of carboxylesterase and inhibition effect of four insecticides on CarE in bifenthrin-resistant population of Aphis sp.
摘要
Abstract
The toxicity of bifenthrin toAphis sp. was determined by the method of the residual film in glass tube. Carboxylesterase activity ofAphis sp. was measured. TheKm andVmax of carboxylesterase in populationⅠ and populationⅤ was studied. And the inhibition effects of 4 insecticides (chlorpyrifos, triazophos, methomyl and omethoate) to CarE in different populations were determined. The results showed thatAphis sp. had resistance to bifenthrin in different levels compared with the sensitive populationⅠ ofAphis sp.. LC50 of the sensitive populationⅠ was 0.057 mg/L, and it was 4.344 mg/L in the case of populationⅤ. The resistance of populationⅤ to bifenthrin was 76.2-fold to that of the sensitive population,which could be classified as high level resistance. The activity of CarE increased with the increasing of resistance levels. There was no significant different inKm between sensitive populationⅠ and high resistant populationⅤ. However, theVmax of resistant populationⅤ was 2.08-fold compared with the sensitive populationⅠ. Inhibition effects of 4 insecticides to CarE of different populations were different, and I50 increased with the increasing of resistance level to bifenthrin. Carboxylesterase plays an important role in the resistance ofAphis sp. to bifenthrin.关键词
杀虫剂/枸杞蚜虫/联苯菊酯/羧酸酯酶/抗性/抑制作用Key words
insecticides/Aphis sp./bifenthrin/carboxylesterase/resistance/inhibition effect分类
农业科技引用本文复制引用
牛婧玮,张丛,常静,李海平,刁青云..抗联苯菊酯枸杞蚜虫羧酸酯酶活性及4种常用杀虫剂对酶的抑制作用[J].农药学学报,2017,19(6):755-759,5.基金项目
国家自然科学基金(31260440). （31260440）
