农药学学报2017，Vol.19Issue(6)：760-764,5.DOI:10.16801/j.issn.1008-7303.2017.0106
高效液相色谱-串联质谱法快速检测马铃薯与土壤中氟醚菌酰胺残留
Determination of LH-2010A residues in potato and soil
冯义志 1李颖 2金杰 1潘金菊 1唐剑峰 2齐晓雪 1梁林 1张爱娟 1刘伟1
作者信息
- 1. 山东省农药科学研究院山东省化学农药重点实验室,济南 250033
- 2. 山东省联合农药工业有限公司,济南 250100
- 折叠
摘要
Abstract
A rapid and sensitive analytical method for the determination of LH-2010A residues in potato and soil was established based on QuEChERS(Quick, Easy, Cheap, Effective, Rugged and Safe) and high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with acetonitrile, and purified with primary secondary amine (PSA) and octadecylsilane (C18). The mass spectrometer was performed in multi-reaction monitoring mode, and electrospray ionization in positive mode was selected. The LH-2010A residues were quantified by the external standard method using the matched standard solution to compensate for matrix effect. The results indicated that the calibration curves of LH-2010A were linear in the range of 0.002-1 mg/L with correlation coefficients of more than 0.9999. When the addition level ranged from 0.005 to 0.5mg/kg,the intra-day average recoveries of LH-2010A in potato, potato plant and soil were 81%-98%, and the relative standard deviation (RSD) ranged from 2.2% to 13% (n = 5). The inter-day average recoveries of LH-2010A were 75%-106%, and with RSD ranged from 0.6% to 11% (n = 15). The limit of quantitation (LOQ) were 0.001 mg/kg in different matrices. This method is simple, rapid and accurate, and can be adapted for the confirmation of LH-2010A residues in potato samples.关键词
高效液相色谱-串联质谱/马铃薯/土壤/杀菌剂/氟醚菌酰胺/残留Key words
high performance liquid chromatography-tandem mass spectrometry/potato/soil/fungicide/LH-2010A/residues分类
化学化工引用本文复制引用
冯义志,李颖,金杰,潘金菊,唐剑峰,齐晓雪,梁林,张爱娟,刘伟..高效液相色谱-串联质谱法快速检测马铃薯与土壤中氟醚菌酰胺残留[J].农药学学报,2017,19(6):760-764,5.
