农药学学报2017，Vol.19Issue(6)：765-770,6.DOI:10.16801/j.issn.1008-7303.2017.0101
柱前衍生-高效液相色谱法检测水中王铜残留的研究
Determination of copper oxychloride residue in water using high performance liquid chromatography method with pre-column derivatization
庾金良 1杜卓时 2周名振 1吕清科 1宋宏宇1
作者信息
- 1. 苏州西山中科药物研究开发有限公司,江苏苏州 215000
- 2. 密苏里大学哥伦比亚分校化学工程系,美国密苏里州哥伦比亚
- 折叠
摘要
Abstract
A new method was developed for the determination of copper oxychloride residue in different water matrix using high performance liquid chromatography with pre-column derivatization. The sample was acidified and then pretreated with pre-column derivatization method using sodium diethyldithiocarbamate before injection. The chromatographic separation was performed on a C18 reversed phase column,using methanol and water as the mobile phase and quantified by UV-vis spectroscopy at the wavelength of 305 nm. The results showed that the calibration curve was linear within the range of 0.06-2 mg/L. The limit of detection (LOD) and limit of quantification (LOQ) of this method were 0.02 mg/L and 0.06 mg/L, respectively. The average recoveries in three different water matrix at three spiked concentration levels of 0.06, 0.2 and 1 mg/L ranged from 85% to 108% with the relative standard deviations (RSD) from 2.2% to 14%. The substances in all different water matrix did not appear to interfere with the determination. This method is simple, accurate and reliable, which is suitable for the rapid determination of copper oxychloride in water.关键词
柱前衍生/高效液相色谱/残留/水/王铜Key words
pre-column derivatization/high performance liquid chromatography/residues/water/copper oxychloride分类
农业科技引用本文复制引用
庾金良,杜卓时,周名振,吕清科,宋宏宇..柱前衍生-高效液相色谱法检测水中王铜残留的研究[J].农药学学报,2017,19(6):765-770,6.
