四川大学学报（自然科学版）2017，Vol.54Issue(2)：381-386,6.DOI:103969/j.issn.0490-6756.2017.03.027
唐古特大黄内生放线菌分离鉴定及生防作用研究
Isolation of endophytic actinomycetes from Rheum tanguticum and its application in biogical control of magnaporthe grisea
摘要
Abstract
Endophytic actinomycetes were isolated from Rheum tanguticum by spread plate method.Inhibitory activities of endophytic actinomycetes were determined by dual-culture inhibition on agar plate and growth rate method.As a result,Strain 4-21with high inhibitory effect on Magnaporthe grisea was found.The results of the inhibiting effects experiment indicated that the strain 4-21 had the strong antagonistic effects,with the inhibition rates of Magnaporthe grisea was 84.12 ％.The strain 4-21 was indentified as Streptomyces albidoflavus according to the morphology biochemical characterization and 16S rDNA gene sequence analysis.The results of potted control effect test showed that the biocontrol efficacy of strain 4-21 to Magnaporthe grisea was 52.73％.Strain 4-21 deserved to be developed a kind of biocontrol agent.关键词
药用植物/内生放线菌/稻瘟病菌/生物防治Key words
Medicinal plant/Endophytic actinomycetes/Magnaporthe grisea/Biological control分类
生物科学引用本文复制引用
廖敏,张波,范中菡,陈熊春蕊,张小平..唐古特大黄内生放线菌分离鉴定及生防作用研究[J].四川大学学报（自然科学版）,2017,54(2):381-386,6.基金项目
国家863计划(2013AA102802-05) （2013AA102802-05）
